LOCUS       sequence-147280-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of codon optimized human NF1 [NM_000267.3] with 
            stop codon (for native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E139 Hs.NF1
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
       61 tctagaggat ctaagcttgc atgcaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttc ctataatgcc aactttgtac aagaaagttg
      181 attacacgat tttcttgatg ctgttccgct tgaagctgcc ggcgcttctc tgcttctgca
      241 cctggctggc gcttccgtgt ctggtcctgc cggagttgca gtggccggtg gtgggagaca
      301 gctccacgtt ttctttgtcg atgccagggc tgtgctgaga tgtggccagg ctggtcatgg
      361 agttggacag gttcaggttg gcggtgatgg acagctggga ctgcagggct ggaggataag
      421 gggaggtggg ggtcagcaga gattcctcgg aggtttcctc gtcgatgccg ggcaggtagg
      481 tatcgatcag ggcgtccagg aacttcacga tcagttcggc gtagtcgggg atctgggtct
      541 gcttgctgaa agggccggca aaccgccaca ggccgttgaa gccgaaggac tgcaggtagc
      601 tggtctggta ctgagggggg ctctcttcgt ggtacaccac actctgcacg atgccgtgga
      661 tgggattcag caggttgggg tcctggcaca gagacagcag ggtgttgatc ttgctatcca
      721 gcagattgtg caccacaggg aacaccttgg gaaacaccac gctggcctcg gccaggtact
      781 cgtacaggat ccgctgatcg aactcgtcgg tggtgtactt caccagggtg gccaggacag
      841 tcagcagc
//
