LOCUS       sequence-147289-         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP10
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctaata cgactcacta tagggagacc
       61 caagctggct agttaagcta tcaacaagtt tgtacaaaaa agttggcatg gccctccaca
      121 gcccgcagta tatttttgga gattttagcc ctgatgaatt caatcaattc tttgtgactc
      181 ctcgatcttc agttgagctt cctccataca gtggaacagt tctgtgtggc acacaggctg
      241 tggataaact acctgatgga caagaatatc agagaattga gtttggtgtc gatgaagtca
      301 ttgaacccag tgacactttg ccgagaaccc ccagctacag tatttcaagc acactgaacc
      361 ctcaggcccc tgaatttatt ctcggttgta cagcttccaa aataacccct gatggtatca
      421 ctaaagaagc aagctatggc tccatcgact gccagtaccc aggctctgcc ctcgctttgg
      481 atggaagttc taatgtggag gcggaagttt tggaaaatga tggtgtctca ggtggtcttg
      541 gacaaaggga gcgtaaaaag aagaaaaagc ggccacctgg atattacagc tatttgaaag
      601 atggtggcga tgatagtatc tccacagaag ccctggtcaa tggccatgcc aattcagcag
      661 tcccgaacag tgtcagtgca gaggatgcag aatttatggg tgacatgcct ccgccactta
      721 cgcccaggac ttgtaacagc ccccagaact ccacagactc tgtcagtgac attgtgcctg
      781 acagtccttt ccccggagca ctcggcagtg acaccaggac tgcagggcag ccagaggggg
      841 g
//
