LOCUS       sequence-147290-         607 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 607)
  AUTHORS   Sowa ME, Bennett EJ, Gygi SP, Harper JW
  TITLE     Defining the human deubiquitinating enzyme interaction landscape.
  JOURNAL   Cell. 2009 Jul 23. 138(2):389-403.
  PUBMED    19615732
REFERENCE   2  (bases 1 to 607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jul 23. 138(2):389-403."
COMMENT     Sequence Label: Flag-HA-USP10
FEATURES             Location/Qualifiers
     source          1..607
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..30)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    62..86
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggccgacttg tcatcgtcgt ccttgtagtc ctcgagttcg aaccgcgggc cctctagatc
       61 aaccactttg tacaagaaag ttggcagcag gtccactcgg cggtaataca ggaggtaggc
      121 tgtgcgttca gcagttggtt tcaccacctg gtactggttg atcaccttga ctgtctggtc
      181 atcgatgcgc agccagccat tcagaccgat ctggaagacg tctgtagtgt aatggccgcc
      241 cgtcgcactg ttgccgtgat ggtagaccac tgcaaagagc cgataggttc ggtggcattt
      301 aaaattctta tttttaaccc ctggagaaag cagttcttta ctaatttcca agtccacagg
      361 atattcaata tttttgataa gcttctggca cccaccagtc ttctcataaa cgaatcgttt
      421 caggtgcagc acgaggacag gagggagttt ttccagagtc actcttcgac ttatctcaac
      481 ctcttgtttg gtttttgtgg tataaccttg gacagattct cttgccacca agctctccag
      541 tgcatcctgg actgtgcgta tcttgtctga ctggatatcc aactgcaacg tgaaaaatgg
      601 ctgcaaa
//
