LOCUS       sequence-147291-         710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human GNPTAB, the alpha/beta subunit 
            precursor miniconstruct that allows its efficient expression, spans 
            the membrane twice, and lacks amino acids 431 to 819. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Marschner K, Kollmann K, Schweizer M, Braulke T, Pohl S
  TITLE     A key enzyme in the biogenesis of lysosomes is a protease that 
            regulates cholesterol metabolism.
  JOURNAL   Science. 2011 Jul 1;333(6038):87-90. doi: 10.1126/science.1205677.
  PUBMED    21719679
REFERENCE   2  (bases 1 to 710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-07-1- 1"; volume: "333"; issue: 
            "6038"; pages: "87-90"
COMMENT     Sequence Label: pcDNA3.1-GNPTAB
FEATURES             Location/Qualifiers
     source          1..710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agttaagctt ggtaccgagc tcggatccag tacccttcac catgctgttc
      121 aagctcctgc agagacagac ctatacctgc ctgtcccaca ggtatgggct ctacgtgtgc
      181 ttcttgggcg tcgttgtcac catcgtctcc gccttccagt tcggagaggt ggttctggaa
      241 tggagccgag atcaatacca tgttttgttt gattcctata gagacaatat tgctggaaag
      301 tcctttcaga atcggctttg tctgcccatg ccgattgacg ttgtttacac ctgggtgaat
      361 ggcacagatc ttgaactact gaaggaacta cagcaggtca gagaacagat ggaggaggag
      421 cagaaagcaa tgagagaaat ccttgggaaa aacacaacgg aacctactaa gaagagtgag
      481 aagcagttag agtgtttgct aacacactgc attaaggtgc caatgcttgt cctggaccca
      541 gccctgccag ccaacatcac cctgaaggac ctgccatctc tttatccttc ttttcattct
      601 gccagtgaca ttttcaatgt tgcaaaacca aaaaaccctt ctaccaatgt ctcagttgtt
      661 gtttttgaca gtactaagga tgttgaagat gcccactctg gactgcttaa
//
