LOCUS       sequence-147303-         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 3 Destination vector - Assembly of 2 Level 2 modules - AB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU3-A-RFP
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             67..78
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    96..112
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(120..126,127..144,145..150))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    165..186
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_feature    311..330
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      333..376
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(380..398)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        466..570
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(789..808)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 accttccata cgaactttga aacgcatgaa ctctttgata acgtcttcgg aggaagccat
       61 ctagtatttc tcctctttct ctagtatgtg tgaaattgtt atccgctcac aattccacac
      121 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
      181 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
      241 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggtc tctatagaga
      301 ttgagacgtt ctagaagcgg ccgcgaattc cagaaatcat ccttagcgaa agctaaggat
      361 tttttttatc tgaaattctg cctcgtgata cgcctatttt tataggttaa tgtcatgata
      421 ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt
      481 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
      541 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
      601 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
      661 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
      721 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
      781 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt
      841 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
      901 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
      961 actgcggcca acttacttct gacaacgatc ggag
//
