LOCUS       sequence-147304-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Level 3 Destination vector - Assembly of 4 Level 2 modules - ABCD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pTU3-B-RFP
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(35..106)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcccacccga aggtgagcca gtgtgactct agtagagagc gttcaccgac aaacaacaga
       61 taaaacgaaa ggcccagtct ttcgactgag cctttcgttt tatttgatgc ctggctctag
      121 tagcgatcta cactagcact atcagcgtta ttaagcaccg gtggagtgac gaccttcagc
      181 acgttcgtac tgttcaacga tggtgtagtc ttcgttgtgg gaggtgatgt ccagtttgat
      241 gtcggttttg taagcacccg gcagctgaac cggtttttta gccatgtagg tggttttaac
      301 ttcagcgtcg tagtgaccac cgtctttcag tttcagacgc attttgattt cacctttcag
      361 agcaccgtct tccgggtaca tacgttcggt ggaagcttcc caacccatgg tttttttctg
      421 cataaccgga ccgtcggacg ggaagttggt accacgcagt ttaactttgt agatgaactc
      481 accgtcttgc agggaggagt cctgggtaac ggtaacaaca ccaccgtctt cgaagttcat
      541 aacacgttcc catttgaaac cttccgggaa ggacagtttc aggtagtccg ggatgtcagc
      601 cgggtgttta acgtaagctt tggaaccgta ctggaactgc ggggacagga tgtcccaagc
      661 gaacggcagc ggaccacctt tggtaacttt cagtttagcg gtctgggtac cttcgtacgg
      721 acgaccttca ccttcacctt cgatttcgaa ctcgtgaccg ttaacggaac cttccatacg
      781 a
//
