LOCUS       sequence-147306-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_H5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 114 to 148)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL4
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     9..27
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(31..74)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    77..96
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        114..148
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(233..261)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 acctataaaa taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg
       61 ctaaggatga tttctggaat tcgcggccgc ttctagaacg tctcaatctc tatttgacgg
      121 ctagctcagt cctaggtaca gtgctagcgg tctcagtaca gatctaataa ttttgtttaa
      181 ctttaaggag atacatatga gaccgcgtaa aggagaagaa cttttcactg gagttgtccc
      241 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      301 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
      361 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag
      421 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      481 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
      541 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
      601 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
      661 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
      721 tggaagcgtt caactagcag accattatca acaa
//
