LOCUS       sequence-147308-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_H5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL4
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(50..107)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(135..162)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(178..249)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(305..325)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaaag cctgcataac gcgaagtaat cttttcggtt
       61 ttaaagaaaa agggcagggt ggtgacacct tgcccttttt tgccggactg cagcgtctct
      121 ggcaaacata taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac tctagtagag
      181 agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact gagcctttcg
      241 ttttatttga tgcctggtcg attatttgta tagttcatcc atgccatgtg taatcccagc
      301 agctgttaca aactcaagaa ggaccatgtg atctctcttt tcgttgggat ctttcgaaag
      361 ggcagattgt gtggacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg
      421 agtattttgt tgataatggt ctgctagttg aacgcttcca tcttcaatgt tgtgtctaat
      481 tttgaagtta actttgattc cattcttttg tttgtctgcc atgatgtata cattgtgtga
      541 gttatagttg tattccaatt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc
      601 ttttaactcg attctattaa caagggtatc accttcaaac ttgacttcag cacgtgtctt
      661 gtagttcccg tcatctttga aaaatatagt tctttcctgt acataacctt cgggcatggc
      721 actcttgaaa aagtcatgct gtttcatatg atctgggtat ctcgcaaagc attgaacacc
      781 ataaccgaaa gtagtgacaa gtgttggcca tggaacaggt agtt
//
