LOCUS       sequence-147309-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Li M, Shin YH, Hou L, Huang X, Wei Z, Klann E, Zhang P
  TITLE     The adaptor protein of the anaphase promoting complex Cdh1 is 
            essential in maintaining replicative lifespan and in learning and 
            memory.
  JOURNAL   Nat Cell Biol. 2008 Sep . 10(9):1083-9.
  PUBMED    19160489
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol. 2008 Sep . 10(9):1083-9."
COMMENT     Sequence Label: FLAG-Ets2
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             79..102
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             111..134
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             144..167
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctacca tggccatgga ctataaggac gatgatgaca aaaagctatg gactataagg
      121 acgatgatga caaaaagcta atggactata aggacgatga tgacaaaaag cttggtaccg
      181 agctcggatc catgaatgat ttcggaatca agaatatgga ccaggtagcc cctgtggcta
      241 acagttacag agggacactc aagcgccagc cagcctttga cacctttgat gggtccctgt
      301 ttgctgtttt tccttctcta aatgaagagc aaacactgca agaagtgcca acaggcttgg
      361 attccatttc tcatgactcc gccaactgtg aattgccttt gttaaccccg tgcagcaagg
      421 ctgtgatgag tcaagcctta aaagctacct tcagtggctt caaaaaggaa cagcggcgcc
      481 tgggcattcc aaagaacccc tggctgtgga gtgagcaaca ggtatgccag tggcttctct
      541 gggccaccaa tgagttcagt ctggtgaacg tgaatctgca gaggttcggc atgaatggcc
      601 agatgctgtg taaccttggc aaggaacgct ttctggagct ggcacctgac tttgtgggtg
      661 acattctctg ggaacatctg gagcaaatga tcaaagaaaa ccaagaaaag acagaagatc
      721 aatatgaaga aaattcacac ctcacctccg ttcctcattg gattaacagc aatacattag
      781 gttttggcac agagcaggcg ccctatggaa tgcagacaca gaattacccc aaaggcggcc
      841 tcctggacag catgtgtccg gcctccacac ccagcgtact cagctctgag caggagtttc
      901 agatgttccc caagtctcgg ctcagctccg tcagcgtcac ctactgctct gtcagtc
//
