LOCUS       sequence-147311-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the S. pyogenes sgRNA from the human U6 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Kabadi AM, Ousterout DG, Hilton IB, Gersbach CA
  TITLE     Multiplex CRISPR/Cas9-based genome engineering from a single 
            lentiviral vector.
  JOURNAL   Nucleic Acids Res. 2014 Aug 13. pii: gku749.
  PUBMED    25122746
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Aug 13. pii: gku749."
COMMENT     Sequence Label: phU6-gRNA
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        complement(50..125)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(144..392)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(202..221)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(372..392)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(443..462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(444..462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(472..489)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(472..488)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(481..503)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(717..736)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aagctggagc tccaccgcgg cgtctcagta cgctcagcgg agaaaaaaag caccgactcg
       61 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttctagctct
      121 aaaacaggtc ttctcgaaga cccggtgttt cgtcctttcc acaagatata taaagccaag
      181 aaatcgaaat actttcaagt tacggtaagc atatgatagt ccattttaaa acataatttt
      241 aaaactgcaa actacccaag aaattattac tttctacgtc acgtattttg tactaatatc
      301 tttgtgttta cagtcaaatt aattccaatt atctctctaa cagccttgta tcgtatatgc
      361 aaatatgaag gaatcatggg aaataggccc tcttcctgcc cgaccttgga tcgatccgcc
      421 tgtgagacgg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
      481 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
      541 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
      601 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
      661 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
      721 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
      781 tcggg
//
