LOCUS       sequence-147312-         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP0
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             74..787
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     CDS             74..787
                     /codon_start=1
                     /product="Aequorea victoria green fluorescent protein"
                     /label=GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(104..132)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     727..747
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggaat tcattaaaga
       61 ggacaaaggt accatgcgta aaggagaaga acttttcact ggagttgtcc caattcttgt
      121 tgaattagat ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga
      181 tgcaacatac ggaaaactta cccttaaatt tatttgcact actggaaaac tacctgttcc
      241 atggccaaca cttgtcacta ctttcggtta tggtgttcaa tgctttgcga gatacccaga
      301 tcatatgaaa cagcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag
      361 aactatattt ttcaaagatg acgggaacta caagacacgt gctgaagtca agtttgaagg
      421 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      481 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      541 acaaaagaat ggaatcaaag ttaacttcaa aattagacac aacattgaag atggaagcgt
      601 tcaactagca gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc
      661 agacaaccat tacctgtcca cacaatctgc cctttcgaaa gatcccaacg aaaagagaga
      721 ccacatggtc cttcttgagt ttgtaacagc tgctgggatt acacatggca tggatgaact
      781 atacaaagcg gcgaacaaaa acgaagaaaa cacc
//
