LOCUS       sequence-147314-         420 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_M2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 102 to 136)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 420)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL5
FEATURES             Location/Qualifiers
     source          1..420
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..62)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    65..84
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        102..136
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(221..249)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
       61 tctggaattc gcggccgctt ctagaacgtc tcaatctcta tttgacggct agctcagtcc
      121 taggtacagt gctagcggtc tcagtacaga tctaataatt ttgtttaact ttggagaaat
      181 acatatgana ccgcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      241 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      301 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      361 gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat acccagatca
//
