LOCUS       sequence-147317-         857 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 113 to 147)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 857)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL8
FEATURES             Location/Qualifiers
     source          1..857
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..26
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(30..73)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    76..95
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        113..147
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(232..260)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 cctataaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct tagctttcgc
       61 taaggatgat ttctggaatt cgcggccgct tctagaacgt ctcaatctct atttgacggc
      121 tagctcagtc ctaggtacag tgctagcggt ctcagtacag atctaataat tttgtttaac
      181 tttaaagaga tacatatgag accgcgtaaa ggagaagaac ttttcactgg agttgtccca
      241 attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt
      301 gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac tggaaaacta
      361 cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg ctttgcgaga
      421 tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga aggttatgta
      481 caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc tgaagtcaag
      541 tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt taaagaagat
      601 ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt atacatcatg
      661 gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa cattgaagat
      721 ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc
      781 cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga tcccaacgaa
      841 aaagagagat cacatgg
//
