LOCUS       sequence-147318-         790 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL8
FEATURES             Location/Qualifiers
     source          1..790
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(23..80)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(108..135)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(151..222)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(278..298)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aagcctgcat aacgcgaagt aatcttttcg gttttaaaga aaaagggcag ggtggtgaca
       61 ccttgccctt ttttgccgga ctgcagcgtc tctggcaaac atataaacgc agaaaggccc
      121 acccgaaggt gagccagtgt gactctagta gagagcgttc accgacaaac aacagataaa
      181 acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctgg tcgattattt
      241 gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa gaaggaccat
      301 gtgatctctc ttttcgttgg gatctttcga aagggcagat tgtgtggaca ggtaatggtt
      361 gtctggtaaa aggacagggc catcgccaat tggagtattt tgttgataat ggtctgctag
      421 ttgaacgctt ccatcttcaa tgttgtgtct aattttgaag ttaactttga ttccattctt
      481 ttgtttgtct gccatgatgt atacattgtg tgagttatag ttgtattcca atttgtgtcc
      541 aagaatgttt ccatcttctt taaaatcaat accttttaac tcgattctat taacaagggt
      601 atcaccttca aacttgactt cagcacgtgt cttgtagttc ccgtcatctt tgaaaaatat
      661 agttctttcc tgtacataac cttcgggcat ggcactcttg aaaaagtcat gctgtttcat
      721 atgatctggg tatctcgcaa agcattgaac accataaccg aaagtagtga caagtgttgg
      781 ccatggaaca
//
