LOCUS       sequence-147319-         877 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 106 to 140)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 877)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL11
FEATURES             Location/Qualifiers
     source          1..877
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..19
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(23..66)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    69..88
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     promoter        106..140
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     primer_bind     complement(225..253)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     848..868
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
       61 gatttctgga attcgcggcc gcttctagaa cgtctcaatc tctatttgac ggctagctca
      121 gtcctaggta cagtgctagc ggtctcagta cagatctaat aattttgttt aactttaaag
      181 ggatacatat gagaccgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg
      241 ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
      301 atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
      361 catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag
      421 atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
      481 gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
      541 gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
      601 ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
      661 aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
      721 ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
      781 cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
      841 atcacatggt ccttcttgag tttgtaacag ctgctgg
//
