LOCUS       sequence-147326-         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create genomic pdt1B 
            insertions. Plasmid contains the R6K origin of replication so needs 
            pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECT1B
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(373..406)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(704..723)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(705..723)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(733..750)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(733..749)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(742..764)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atcatgcgaa acgatcctca tcctgtctct tgatcagatc ttgatcccct gcgccatcag
       61 atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac cttaccagag
      121 ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca gtctagctat
      181 cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt ttcccttgtc
      241 cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg actggctttc
      301 tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca gcgtgagctt
      361 caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcgaac tgcaggtcga
      421 cggatccccg gaatcatggt actttctcct ccgcggttac aactgaagac ggttcgcctg
      481 gcccgcgttc agcatgatcg cgcgcacttc gttggtgttt tcttcgtttt tgttcgccgc
      541 ctgcccgctt tccagtcggg aaacctgtcg tgccagttgc attaatgaat cggccaacgc
      601 gcggggagag gcggtttgcg tattgggagc cagggtggtt tttcttttca ccagtgagac
      661 gggcaacagc tgattctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
      721 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaa
//
