LOCUS       sequence-147327-         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L1U1H08 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L1U1H08
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     8..25
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(49..106)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    199..218
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      221..264
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(268..286)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(514..533)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 cgagcgcagc gagtcagtga gcgaggaagc ctgcataacg cgaagtaatc ttttcggttt
       61 taaagaaaaa gggcagggtg gtgacacctt gccctttttt gccggactgc agctaatgag
      121 acggcatgcg gtctctaaca aaaaaaaaac ccgcatgcga acatgcgggt cgatgagacc
      181 catatgcgtc tctagattct agaagcggcc gcgaattcca gaaatcatcc ttagcgaaag
      241 ctaaggattt tttttatctg aaattctgcc tcgtgatacg cctattttta taggttaatg
      301 tcatgataat aatggtttct tagacgtcag gtggcactcg agttgatcgg gcacgtaaga
      361 ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg
      421 agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt
      481 gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt
      541 acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat
      601 aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg
      661 gaatttcgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt
      721 tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac
      781 gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaaacct
      841 ggcctatttc cctaaagggt ttattgagaa tatgtt
//
