LOCUS       sequence-147329-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses the acyl carrier protein (ACP) from the E. coli fatty acid
            synthase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Worthington AS, Rivera H, Torpey JW, Alexander MD, Burkart MD
  TITLE     Mechanism-based protein cross-linking probes to investigate carrier 
            protein-mediated biosynthesis.
  JOURNAL   ACS Chem Biol. 2006 Dec 20;1(11):687-91.
  PUBMED    17184132
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Chem 
            Biol."; date: "2006-12-20"; volume: "1(11)"; pages: "687-91"
COMMENT     Sequence Label: AcpP pet29a C-terminal His Tag
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(45..275)
                     /codon_start=1
                     /gene="acpP"
                     /product="acyl carrier protein (ACP) from E. coli"
                     /label=ACP
                     /translation="STIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELVMA
                     LEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     CDS             complement(294..311)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(327..371)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     RBS             382..404
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    419..443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(443..462)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(444..462)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(550..569)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagcgcctg gtggccgttg
       61 atgtaatcaa tggcagcctg aacggtggtg attttctcag cttcttcgtc cggaatctca
      121 gtatcaaact cttcttccag agccattacc agctcaacgg tgtcaagaga atccgcgccc
      181 aggtcttcaa cgaaagaagc attgttggta acttcttcct gcttaacgcc cagctgttcg
      241 ccgataattt tcttaacgcg ttcttcgata gtgctggatc cgatatcagc catggaaccg
      301 cgtggcacca gggtacccag atctgggctg tccatgtgct ggcgttcgaa tttagcagca
      361 gcggtttctt tcatatgtat atctccttct taaagttaaa caaaattatt tctagagggg
      421 aattgttatc cgctcacaat tcccctatag tgagtcgtat taatttcgcg ggatcgagat
      481 cgatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
      541 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
      601 tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg
      661 gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac
      721 tactgggctg cttcctaatg caggagtcgc ataagg
//
