LOCUS       sequence-147332-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid that serves as the PCR template to create the genomic pdt1 
            insertions contained in the EPD library. Plasmid contains the R6K 
            origin of replication so needs pir gene to replicate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECM1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(387..420)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(724..743)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(725..743)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
      181 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
      241 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
      301 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc
      361 ggcagcgtga gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      421 gaactgcagg tcgacggatc cccggaatca tggtactttc tcctccgcgg ttacaactga
      481 agacggttcg cctggcccgc gttcagcata aaggtcggca cttcgttggt gttttcttcg
      541 tttttgttcg ccgctccaac ctgcccgctt tccagtcggg aaacctgtcg tgccagttgc
      601 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggagc cagggtggtt
      661 tttcttttca ccagtgagac gggcaacagc tgattctcga gggggggccc ggtacccaat
      721 tcgccctata gtgagtcgta ttacgcgcgc t
//
