LOCUS       sequence-147341-         792 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toggle switch plasmid containing lacI-pdt3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 792)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECJ3B
FEATURES             Location/Qualifiers
     source          1..792
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    152..168
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(176..182,183..199,200..205))
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 atctcgaact cgtggccgtt cacggagccc tccatgtgaa ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca tcggcgcctc cctatcagtt
      121 gaacgatagt taatactcaa ttggtgtgaa attgttatcc gctcacaatt ccacacatta
      181 tacgagccgg atgattaatt gtcaacagct catttcgccc gggaacgagc atatggagcg
      241 acttaagcag tccgagctct caagacccac tttcacattt aagttgtttt tctaatccgc
      301 agatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt
      361 cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt
      421 tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc
      481 tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat
      541 tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat
      601 cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc
      661 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
      721 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
      781 ctagcttctg gg
//
