LOCUS       sequence-147343-         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Toggle switch plasmid containing lacI-pdt3B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECJ3B
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(234..252,253..258,259..275,276..281,282..307)
                     /label=PLtetO-1 promoter
                     /note="modified phage lambda PL promoter with tet operator 
                     sites (Lutz and Bujard, 1997)"
     protein_bind    234..252
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(389..417)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaagaaaaac caccctggct cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt
       61 cattaatgca actggcacga caggtttccc gactggaaag cgggcaggcg gcgaacaaaa
      121 acgaagaaaa caccaacgaa gtgcgcgcga tcatgctgaa cgcgggccag gcgaacagaa
      181 gacgagttta accgcggcaa agtggtacct tgcggaagct tctgcgaaac gtttccctat
      241 cagtgataga gattgacatc cctatcagtg atagagatac tgagcacatc agcaggacgc
      301 actgaccgca tgccagttgt agatcagcat agcatagaca accaaggagg tagtgcacat
      361 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga
      421 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa
      481 acttaccctt aaatttattt gcactactgg aaaactacct gttccgtggc caacacttgt
      541 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcaca tgaaacagca
      601 tgactttttc aagagtgcca tgcccgaagg ttacgtacag gaaagaacta tatttttcaa
      661 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa
      721 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt
      781 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat
      841 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca
      901 ttatcaacaa aatactccga ttggcgatgg ccctgtc
//
