LOCUS       sequence-147350-         458 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP4
FEATURES             Location/Qualifiers
     source          1..458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(146..232)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(399..419)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
       61 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
      121 gaacgcccta ggtctagggc ggcggatttg tcctactcag gagagcgttc accgacaaac
      181 aacagataaa acgaaaggcc cagtctttcg actgagcctt tcgttttatt tgatgcctct
      241 agcacgcgta ccatgggatc ccccatcaag cttattaagg ttgggcgtgg ttcgcctggc
      301 ccgcgttcag cataaaggtc ggcacttcgt tggtgttttc ttcgtttttg ttcgccgctt
      361 tgtatagttc atccatgcca tgtgtaatcc cagcagctgt tacaaactca agaaggacca
      421 tgtggtctct cttttcgttg ggatctttcg aaagggca
//
