LOCUS       sequence-147351-        1001 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1001)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1001)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His10 TEV LIC cloning vector (2B-T-10)
FEATURES             Location/Qualifiers
     source          1..1001
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             72..94
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             114..143
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             144..164
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     primer_bind     complement(289..307)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      303..350
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        complement(join(716..721,722..738,739..744))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     primer_bind     complement(771..789)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        857..961
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cctctagtgc cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt
       61 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc
      121 accatcacca tcaccatcac catgaaaacc tgtacttcca atccaatatt ggaagtggat
      181 aacggatccg cgatcgcggc gcgccacctg gtggccggcc ggtaccacgc gtgcgcgctg
      241 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
      301 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag
      361 gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg
      421 gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg
      481 agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga
      541 ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc
      601 aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta
      661 gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa
      721 agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat
      781 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      841 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      901 gtatccgctc atgagacaat aaccctgata aatgcttcaa taacattgaa aaaggaagag
      961 tatgagtatt caacatttcc gtgtcgccct tattcccttt t
//
