LOCUS       sequence-147356-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  TITLE     2-series LIC N-terminal fusion vectors for E. coli expression
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pET His10 TEV LIC cloning vector (2B-T-10)
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..129)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(130..159)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     RBS             179..201
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(289..308)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(290..308)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(392..411)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 agcttccttt cgggctttgt tagcagccgg atcagcgcgc acgcgtggta ccggccggcc
       61 accaggtggc gcgccgcgat cgcggatccg ttatccactt ccaatattgg attggaagta
      121 caggttttca tggtgatggt gatggtgatg gtgatggtga gaagatttca tatgtatatc
      181 tccttcttaa agttaaacaa aattatttct agaactcgag tcctgcaggg cggccgctta
      241 attaaagagc tctccggagc cggcactaga gggaaaccgt tgtggtctcc ctatagtgag
      301 tcgtattaat ttcgcgggat cgagatctcg atcctctacg ccggacgcat cgtggccggc
      361 atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac cgatggggaa
      421 gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc
      481 cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct tgcggcggcg
      541 gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc gcataaggga
      601 gagcgtcgac cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg
      661 ggcatgacta tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag
      721 gtgccggcag cgctctgggt cattttcggc gaggaccgct ttcgctggag cgcgacgatg
      781 atcggcctgt cgcttgcggt attcggaatc ttgcacgccc tcgctcaagc cttcgtcact
      841 ggtcccgcca ccaaacgttt cggcgagaag caggccatta tcgccggcat ggcggccgac
      901 gcgctgggct acgtcttgct ggcgttcgcg acgcgaggct ggatggcctt ccccattatg
      961 attcttctcg cttccggcgg catcgggatg cccgcgttgc aggccatgct gtccaggcag
     1021 gtagatgacg accatcaggg acagcttcaa gga
//
