LOCUS       sequence-147361-         650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of mcherry-pdt3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27MC3
FEATURES             Location/Qualifiers
     source          1..650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(214..300)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
       61 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      121 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      181 ccgcagccga acgccctagg tctagggcgg cggatttgtc ctactcagga gagcgttcac
      241 cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc gttttatttg
      301 atgcctctag cacgcgtacc atgggatccc ccatcaagct tattaaactc gtcttctgtt
      361 cgcctggccc gcgttcagca taaaggtcgg cacttcgttg gtgttttctt cgtttttgtt
      421 cgccgccttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg cgcgttcgta
      481 ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga tgttgacgtt
      541 gtacgcgccg ggcagttgca cgggcttctt ggccttgtag gtggtcttga cctcagcgtc
      601 gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
//
