LOCUS       sequence-147370-         467 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 467)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP3D
FEATURES             Location/Qualifiers
     source          1..467
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(155..241)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(408..428)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
       61 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      121 gccgcagccg aacgccctag gtctagggcg gcggatttgt cctactcagg agagcgttca
      181 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      241 gatgcctcta gcacgcgtac catgggatcc cccatcaagc ttattaaact cgtcttctgt
      301 tcgcctggcc cgcgttcagc atattggggg ccacttcgtt ggtgttttct tcgtttttgt
      361 tcgccgcttt gtatagttca tccatgccat gtgtaatccc agcagctgtt acaaactcaa
      421 gaaggaccat gtggtctctc ttttcgttgg gatctttcga aagggca
//
