LOCUS       sequence-147378-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive expression of gfp-pdt2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZE27GFP2
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(197..283)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(450..470)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
       61 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
      121 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgccct
      181 aggtctaggg cggcggattt gtcctactca ggagagcgtt caccgacaaa caacagataa
      241 aacgaaaggc ccagtctttc gactgagcct ttcgttttat ttgatgcctc tagcacgcgt
      301 accatgggat cccccatcaa gcttattatt gagacaggta gttcgcctgg cccgcgttca
      361 gcataaaggt cggcacttcg ttggtgtttt cttcgttttt gttcgccgct ttgtatagtt
      421 catccatgcc atgtgtaatc ccagcagctg ttacaaactc aagaaggacc atgtggtctc
      481 tcttttcgtt gggatctttc gaaagggcag attgtgtgga caggtaatgg ttgtctggta
      541 aaaggacagg gccatcgcca attggagtat tttgttgata atggtctgct agttgaacgc
      601 ttccatcttc aatgttgtgt ctaattttga agttaacttt gattccattc ttttgtttgt
      661 ctgccatgat gtatacattg tgtgagttat agttgtattc caa
//
