LOCUS       sequence-147379-        1000 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GST tagged GINA3 protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, 
            Kufareva I, Abagyan R, Ghosh P
  TITLE     Structural basis for activation of trimeric Gi proteins by multiple 
            growth factor receptors via GIV/Girdin.
  JOURNAL   Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 
            10.1091/mbc.E14-05-0978. Epub 2014 Sep 3.
  PUBMED    25187647
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-05-0978"; journalName: "Mol Biol Cell"; date: 
            "2014-11-5- 5"; volume: "25"; issue: "22"; pages: "3654-71"
COMMENT     Sequence Label: GST-GINA3
FEATURES             Location/Qualifiers
     source          1..1000
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(205..309)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     377..395
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     540..562
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
       61 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
      121 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
      181 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
      241 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
      301 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat
      361 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aattatacac
      421 tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga
      481 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
      541 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagatcgt
      601 cagtcagtca cgatgcggcc gctcgagtcg actcaataaa gtccacattc ctttaagttg
      661 tttttaatga tgacatctgt aacagcatca aaaacaaact gcacgttttt ggtgtcagtg
      721 gcacaggtaa agtgagtgta gacctccttg gtgtcctttc ttcggttcag atcttcaaac
      781 tggcactgga tgtaagcagc tgcctcttcg tacgtattgg aacctgtgta ttctggataa
      841 cagattgtta atggactcct ctttattttt tcctcaaaaa ggtctttctt attaaggaag
      901 agaatgattg aagtgtctgt aaaccatttg ttgttacaaa tgctgtcaaa caatttcatg
      961 ctttcatgca ttcggttcat ttcctcgtcc tcagccagaa
//
