LOCUS       sequence-147385-         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for building 5n1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Niederholtmeyer H, Sun ZZ, Hori Y, Yeung E, Verpoorte A, Murray RM, 
            Maerkl SJ
  TITLE     Rapid cell-free forward engineering of novel genetic ring 
            oscillators.
  JOURNAL   Elife. 2015 Oct 2;4. doi: 10.7554/eLife.09771.
  PUBMED    26430766
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-10-2"; volume: "4. doi"; pages: " 10.7554/eLife.09771"
COMMENT     Sequence Label: pCI-BCD7-lacI-ssrA P62U18C50T16 v40-4a (496)
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(263..282)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag cgaagacaag
       61 gttgcattgc tgttcctgca ggtcgaggat aaatatctaa caccgtgcgt gttgactatt
      121 ttacctctgg cggtgataat ggttgcatgt actaaagcgg gcccaagttc acttaaaaag
      181 gagatcaaca atgaaagcaa ttttcgtact gaaacatctt aatcatgctg gggagggttt
      241 ctaatgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc
      301 gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa
      361 gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa
      421 cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt
      481 gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta
      541 gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctagc gcaacgcgtc
      601 agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc
      661 tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt
      721 attttctccc atgaggacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac
      781 cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct
      841 ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
//
