LOCUS       sequence-147389-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: N1 Cloning Vector, Excitation: 559, Emission: 600.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Lam AJ, St-Pierre F, Gong Y, Marshall JD, Cranfill PJ, Baird MA, 
            McKeown MR, Wiedenmann J, Davidson MW, Schnitzer MJ, Tsien RY, Lin 
            MZ
  TITLE     Improving FRET dynamic range with bright green and red fluorescent 
            proteins.
  JOURNAL   Nat Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171.
  PUBMED    22961245
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2012 Sep 9. doi: 10.1038/nmeth.2171."
COMMENT     Sequence Label: mRuby2-N1
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    25..105
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      107..116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg agctcaagct
       61 tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgtc
      121 taagggcgaa gagctgatca aggaaaatat gcgtatgaag gtggtcatgg aaggttcggt
      181 caacggccac caattcaaat gcacaggtga aggagaaggc aatccgtaca tgggaactca
      241 aaccatgagg atcaaagtca tcgagggagg acccctgcca tttgcctttg acattcttgc
      301 cacgtcgttc atgtatggca gccgtacttt tatcaagtac ccgaaaggca ttcctgattt
      361 ctttaaacag tcctttcctg agggttttac ttgggaaaga gttacgagat acgaagatgg
      421 tggagtcgtc accgtcatgc aggacaccag ccttgaggat ggctgtctcg tttaccacgt
      481 ccaagtcaga ggggtaaact ttccctccaa tggtcccgtg atgcagaaga agaccaaggg
      541 ttgggagcct aatacagaga tgatgtatcc agcagatggt ggtctgaggg gatacactca
      601 tatggcactg aaagttgatg gtggtggcca tctgtcttgc tctttcgtaa caacttacag
      661 gtcaaaaaag accgtcggga acatcaagat gcccggtatc catgccgttg atcaccgcct
      721 ggaaaggtta gaggaaagtg acaatgaaat gttcgtagta caacgcgaac acgcagttgc
      781 caagttcgcc gggcttggtg gtggg
//
