LOCUS       sequence-147398-         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral expression plasmid for human NFATc2/C (with RIT mutation 
            abrogating AP1 binding) with C-terminal BirA-biotinylation signal 
            and TEV celavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(12..28)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13..29)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     CDS             complement(68..112)
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             complement(125..145)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(152..175)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcaagctta tcgataccgt cgacctcgac ctcgacgcgg ccgcttataa gcgatcgctc
       61 agttaacctc atgccattcg attttctgtg cctcgaagat atcgttcagg ccaccgccgt
      121 taacgccctg gaagtacagg ttctctacaa gcttatcgtc gtcatccttg taatctacgt
      181 acgtctgatt tctggcagga ggtcctgaaa actccttcct gataatttca ttaacatcat
      241 ccaagtaggt ctggtccaag ttctgctcct gtttaatggt cacccccgca ggtaatactt
      301 ccttttggtc actgaccggg ggtccgtttt tggcggctct ttggctcgtg gcattctgct
      361 gctgaatgac tgtggggtag gaacccgggc tcagcctctg accttgactg accgggggcg
      421 ggccaggtct ggtggtgcct ggtgcgaaat tctcgcagta catgatgtgc tggaactcct
      481 ggtggcttcc gcagcgcagc tgctggttgg tgggtgagta gtggatcaca ggcgaggcct
      541 gctggttggt cggagagggg tggagcaggg ctga
//
