LOCUS       sequence-147399-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for arabinose-induced expression of mf-lon. Note that this 
            plasmid contains the original mf-lon cassette that is not codon 
            optimized for expression in E. coli..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pZA16mflon
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             31..42
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
ORIGIN
        1 actctctact gtttctccat agaattcatt aaagaggaga aaggtaccat gagtaaaaaa
       61 ataaaactac ctattttcca aatacgcggt tcatttattg ttcctggaat taaagagaat
      121 ttagaagttg gaagaaaaaa cacattagcg agcgttaatt atgcgattaa aaattcaaat
      181 aatcaaatga tcgcaatacc gcagattgat gcatctgttg aaaaacctga attttcagat
      241 ttacatgaat ttggaatttt aattgatttt gaagtaatca aagaatggaa agataattca
      301 ttaactatta gcacaaaccc tattcaacga tgtaaagtaa ttagcttttt tgaaaatgaa
      361 gatcaagtgc cgtatgcgga agttgaatta attgaatcaa ttaatgattt tagtgatgaa
      421 gagttaaaag aattaattga aaaaatctct gatgctatca aaactaaagc tagtttagta
      481 acaaaacaaa ttaaacagtt aatatcaggt gaatctgatg atttaagttt agcttttgat
      541 tcaattatgt ttaaactggc tccgtcaaaa atattgacaa atccggaata cataacatct
      601 ccgtcattaa aaacacgatg gtcaattatt gaaaaaataa tttttgctga agatggaatc
      661 ataacaagaa atgcagaatc aattgatgca gcacgac
//
