LOCUS       sequence-147404-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His tagged GINA3 protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Lin C, Ear J, Midde K, Lopez-Sanchez I, Aznar N, Garcia-Marcos M, 
            Kufareva I, Abagyan R, Ghosh P
  TITLE     Structural basis for activation of trimeric Gi proteins by multiple 
            growth factor receptors via GIV/Girdin.
  JOURNAL   Mol Biol Cell. 2014 Nov 5;25(22):3654-71. doi: 
            10.1091/mbc.E14-05-0978. Epub 2014 Sep 3.
  PUBMED    25187647
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-05-0978"; journalName: "Mol Biol Cell"; date: 
            "2014-11-5- 5"; volume: "25"; issue: "22"; pages: "3654-71"
COMMENT     Sequence Label: His-GINA3
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(28..75)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     71..89
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(142..159)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caatccggat atagttcctc ctttcagcaa aaaacccctc aagacccgtt tagaggcccc
       61 aaggggttat gctagttatt gctcagcggt ggcagcagcc aactcagctt cctttcgggc
      121 tttgttagca gccggatctc agtggtggtg gtggtggtgc tcgagtgcgg ccgcaagctt
      181 gtcgacggag ctcgaattcg gatcctcagt aaagcccaca ttcctttaag ttgtttttaa
      241 tgatgacatc tgtaacagca tcaaaaacaa actgcacgtt tttggtgtca gtggcacagg
      301 taaagtgagt gtagacctcc ttggtgtcct ttcttcggtt cagatcttca aactggcact
      361 ggatgtaagc agctgcctct tcgtacgtat tggaacctgt gtattctgga taacagattg
      421 ttaatggact cctctttatt ttttcctcaa aaaggtcttt cttattaagg aagagaatga
      481 ttgaagtgtc tgtaaaccat ttgttgttac aaatgctgtc aaacaatttc atgctttcat
      541 gcattcggtt catttcctcg tcctcagcca gaacaaggtc gtaatcactg agagccacac
      601 aaaagataat tgctgtcact ccctcaaaac agtggatcca ctttttccgt tcggatcttt
      661 ggccacctac atcaaacatt ttgaagtaga gctccttgaa ggtgaagtgg gtctccacaa
      721 tgcctgtagt cttcactctc gtccgaagaa catcttgctg agttggaatg tagttggtct
      781 gggatattct atccaaatca tttaggtaat atgaagcaga atcattgagc tgatattccc
      841 tggacctgct gaagcatgcc tgcaccccgc catctcgcca taaacgttta atcacgcctg
      901 ctagttctga agtcatgact ccttcctcag cactgccagc taaaacaaat aactgtcggg
//
