LOCUS       sequence-147417-         736 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for mf-lon mediated mCherry degradation in Lactococcus 
            lactis. Contains mcherry-pdt3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 736)
  AUTHORS   Cameron DE, Collins JJ
  TITLE     Tunable protein degradation in bacteria.
  JOURNAL   Nat Biotechnol. 2014 Dec;32(12):1276-81. doi: 10.1038/nbt.3053. Epub
            2014 Nov 17.
  PUBMED    25402616
REFERENCE   2  (bases 1 to 736)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3053"; 
            journalName: "Nat Biotechnol"; date: "2014-12"; volume: "32"; issue:
            "12"; pages: "1276-81"
COMMENT     Sequence Label: pECGMC3
FEATURES             Location/Qualifiers
     source          1..736
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(179..206)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctccttctta attacaaatt tttagcatct aatttaactt caattcctat tatacaaaat
       61 tttaagatac tgcactatca acacactctt aagtttgctt ctaagtctta tttccataac
      121 ttcttttacg tttccgcagg ctgcaggcgg atccccaaca agagtttgta gaaacgcaaa
      181 aaggccatcc gtcaggatgg ccttctgctt agctagagcg gcggatttgt cctactcagg
      241 gacgtcttat tttgttttaa aaactattgc aaatacctct tcatattttt cagcaggtat
      301 aatttttagt tttgctttta cttcatctgg aatttcgtct aagtctcttt catttttctt
      361 tggaataatg attgttttca atccacttct tgaagcagaa attgattttt ctcttaatcc
      421 accaattggc aatacatttc ctcttagtgt gatttcacca gtcattccaa tttcttttga
      481 aactggttta tctgaaagtg cagaaattaa agcagttgta attgtgatac cagcacttgg
      541 tccatctttt ggaacagctc cttcaggaac gtgaacatga atatcatttt cttcaaatac
      601 ttttttatct actccaaatt tttcaaagtt tgatttaaca taagttaacg caattgttgc
      661 agattctttc ataacttcac caagtttacc tgtaagaatt aaatttcctt taccagggta
      721 taaacttact tcaatt
//
