LOCUS       sequence-147426-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Darzacq X, Shav-Tal Y, de Turris V, Brody Y, Shenoy SM, Phair RD, 
            Singer RH
  TITLE     In vivo dynamics of RNA polymerase II transcription.
  JOURNAL   Nat Struct Mol Biol. 2007 Sep;14(9):796-806. Epub 2007 Aug 5.
  PUBMED    17676063
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Mol Biol."; date: "2007-09"; volume: "14(9)"; pages: "796-806. Epub 
            2007 Aug 5"
COMMENT     Sequence Label: pYFP-RPB1aAmr
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(22..43)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(283..302)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     630..651
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
       61 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      121 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc
      181 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      241 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
      301 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
      361 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
      421 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
      481 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
      541 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg
      601 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
      661 gggatcactc tcggcatgga cgagctgt
//
