LOCUS       sequence-147440-         521 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding a human BRD9 bromodomain-swap
            mutant containing the first bromodomain of BRD4 with a C terminal 3x
            FLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..521
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(59..124)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 aagcgcatgc tccagactgc cttgggaaaa gcgcctcccc tacccggtag aattctcact
       61 tgtcatcgtc atccttgtaa tcgatatcat gatctttata atcaccgtca tggtctttgt
      121 aatcgcctcc ggtcttggca gaggccgcag gctctggaga ctgaagaaac tcataggggt
      181 cgtgggtgac gtctggctgc tccccgacac tcaggcgaga agggcttccc aggtggtgct
      241 ggtccctctc ggaggcgttg gacagggagc tgaggttgga cgacggccga gagccgccgc
      301 gctccgcctg tgcttcgtgc aggtcctgca ggagcttcgt cgtctcatcc aagttcaaat
      361 ggctgtcgtc agggtccagc tccttcttca ccttccccag agagctgagc atggagatat
      421 ccacagaaac gtcgggatag gacttcatcg acatgaactc cagaacagag ctgctgctgt
      481 ctcctagggt gtccccaacc ttggcttcat ctggaggctt c
//
