LOCUS       sequence-147441-         753 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding a human BRD9 mutant carrying 
            the N216A single amino acid mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-BRD9_N216A-PGK-Puro-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(476..487)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 agaggccgca ggctctggag actgaagaaa ctcatagggg tcgtgggtga cgtctggctg
       61 ctccccgaca ctcaggcgag aagggcttcc caggtggtgc tggtccctct cggaggcgtt
      121 ggacagggag ctgaggttgg acgacggccg agagccgccg cgctccgcct gtgcttcgtg
      181 caggtcctgc aggagcttcg tcgtctcatc caagttcaaa tggctgtcgt cagggtccag
      241 ctccttcttc accttcccca gagagctgag catggagata tccacagaaa cgtcgggata
      301 ggacttcatc gacatgaact ccagaacaga gctgctgctg tctcctaggg tgtccccaac
      361 cttggcttca tctggaggct tcatgggaac atttcttctc tgcttcagct ggaagagcgt
      421 cctagagtgg tctccgcctg tgatctggtc caggaggtcg tccaccactt tcttgctgta
      481 gctcccagca tccttcacaa actcctgcag gctcagcgca cactgcacgc ctgtctcatc
      541 tccgtaggct gagtagagca gctccatctc gtccgacttc aagtcgccaa atactgaatt
      601 attctgcatc gaaagcgcag tagtggcact ggagagaaag gtgactttgt ttcttctctc
      661 gtctttgaag cccagcgtgg tgaagcctgg gagtagctta ctggagagcg agctcaagtc
      721 caccgggtgg gtctcctcct catcagcgtc cgg
//
