LOCUS       sequence-147451-       10644 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Circuit plasmid: 0xF6.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10644)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 10644)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN3938
FEATURES             Location/Qualifiers
     source          1..10644
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(308..852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(388..407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1756)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(941..1756)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1664..1683
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1748..1767
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        join(2837..2842,2843..2858,2859..2865)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    2871..2890
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     protein_bind    2911..2929
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2936..2954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4700..4718
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4725..4743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(5596..5601,5602..5617,5618..5624)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    5630..5649
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        6457..6741
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     6664..6683
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     promoter        7622..7656
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             7665..7676
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8532..8543
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8661..9743
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8680..8699)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             9762..10385
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(9835..9854)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10568..10587)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcctcgc tcactgactc gctgcacgag gcagacctca gcgctagcgg agtgtatact
       61 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      121 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      181 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      241 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      301 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      361 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      421 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      481 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      541 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      661 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      721 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      781 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
      841 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac tcatcgagca
      961 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1021 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1081 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1141 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1201 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1261 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1321 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1381 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1441 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     1501 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     1561 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     1621 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     1681 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     1741 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     1801 ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag acacaaccaa
     1861 ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctcccg ctttctatga
     1921 ttggtccaga ttcgttacca attgacagct agctcagtcc taggtatata catacatgct
     1981 tgtttgtttg taaacagcgc gggtgagagg gattcgttac caattgacaa ttgattggac
     2041 gttcaatata atgctagcct gaagcggtca acgcatgtgc tttgcgttct gatgagacag
     2101 tgatgtcgaa accgcctcta caaataattt tgtttaactt tacgagggcg atcctatggc
     2161 acgtaccccg agccgtagca gcattggtag cctgcgtagt ccgcataccc ataaagcaat
     2221 tctgaccagc accattgaaa tcctgaaaga atgtggttat agcggtctga gcattgaaag
     2281 cgttgcacgt cgtgccggtg caagcaaacc gaccatttat cgttggtgga ccaataaagc
     2341 agcactgatt gccgaagtgt atgaaaatga aagcgaacag gtgcgtaaat ttccggatct
     2401 gggtagcttt aaagccgatc tggattttct gctgcgtaat ctgtggaaag tttggcgtga
     2461 aaccatttgt ggtgaagcat ttcgttgtgt tattgcagaa gcacagctgg accctgcaac
     2521 cctgacccag ctgaaagatc agtttatgga acgtcgtcgt gagatgccga aaaaactggt
     2581 tgaaaatgcc attagcaatg gtgaactgcc gaaagatacc aatcgtgaac tgctgctgga
     2641 tatgattttt ggtttttgtt ggtatcgcct gctgaccgaa cagctgaccg ttgaacagga
     2701 tattgaagaa tttaccttcc tgctgattaa tggtgtttgt ccgggtacac agcgttaagg
     2761 aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggtgtca
     2821 acgatcgttg gctgtgttga caattaatca tcggctcgta taatgtgtgg aattgtgagc
     2881 gctcacaatt tactccaccg ttggcttttt tccctatcag tgatagagat tgacatccct
     2941 atcagtgata gagataatga gcacctgaag cgctcaacgg gtgtgcttcc cgttctgatg
     3001 agtccgtgag gacgaaagcg cctctacaaa taattttgtt taagagtcta tggactatgt
     3061 tttcacaaag gaagtaccag gatggcacgt aaaaccgcag cagaagcaga agaaacccgt
     3121 cagcgtatta ttgatgcagc actggaagtt tttgttgcac agggtgttag tgatgcaacc
     3181 ctggatcaga ttgcacgtaa agccggtgtt acccgtggtg cagtttattg gcattttaat
     3241 ggtaaactgg aagttctgca ggcagttctg gcaagccgtc agcatccgct ggaactggat
     3301 tttacaccgg atctgggtat tgaacgtagc tgggaagcag ttgttgttgc aatgctggat
     3361 gcagttcata gtccgcagag caaacagttt agcgaaattc tgatttatca gggtctggat
     3421 gaaagcggtc tgattcataa tcgtatggtt caggcaagcg atcgttttct gcagtatatt
     3481 catcaggttc tgcgtcatgc agttacccag ggtgaactgc cgattaatct ggatctgcag
     3541 accagcattg gtgtttttaa aggtctgatt accggtctgc tgtatgaagg tctgcgtagc
     3601 aaagatcagc aggcacagat tatcaaagtt gcactgggta gcttttgggc actgctgcgt
     3661 gaaccgcctc gttttctgct gtgtgaagaa gcacagatta aacaggtgaa atccttcgaa
     3721 taattcagcc aaaaaactta agaccgccgg tcttgtccac taccttgcag taatgcggtg
     3781 gacaggatcg gcggttttct tttctcttct caagggcacc aggaatctga acgattcgtt
     3841 accaattgac atatttaaaa ttcttgttta aaatgctagc tcgtcactag agggcgatag
     3901 tgacaaactt gacaactcat cacttcctag gtataatgct agcctgaaga agtcaattaa
     3961 tgtgctttta attctgatga gtcggtgacg acgaaacttc ctctacaaat aattttgttt
     4021 aaccccccga ggagtagcac atgccgaaac tgggtatgca gagcattcgt cgtcgtcagc
     4081 tgattgatgc aaccctggaa gcaattaatg aagttggtat gcatgatgca accattgcac
     4141 agattgcacg tcgtgccggt gttagcaccg gtattattag ccattatttc cgcgataaaa
     4201 acggtctgct ggaagcaacc atgcgtgata ttaccagcca gctgcgtgat gcagttctga
     4261 atcgtctgca tgcactgccg cagggtagcg cagaacagcg tctgcaggca attgttggtg
     4321 gtaattttga tgaaacccag gttagcagcg cagcaatgaa agcatggctg gcattttggg
     4381 caagcagcat gcatcagccg atgctgtatc gtctgcagca ggttagcagt cgtcgtctgc
     4441 tgagcaatct ggttagcgaa tttcgtcgtg aactgcctcg tgaacaggca caagaggcag
     4501 gttatggtct ggcagcactg attgatggtc tgtggctgcg tgcagcactg agcggtaaac
     4561 cgctggataa aacccgtgca aatagcctga cccgtcattt tatcacccag catctgccga
     4621 ccgattaacc aattattgaa cacccttcgg ggtgtttttt tgtttctggt ctaccgtaat
     4681 actccaccgt tggctttttt ccctatcagt gatagagatt gacatcccta tcagtgatag
     4741 agataatgag cacctgaagg ggtcagttga tgtgctttca actctgatga gtcagtgatg
     4801 acgaaacccc ctctacaaat aattttgttt aactatggac tatgttttca catacgaggg
     4861 ggattagatg aacaaaacca ttgatcaggt gcgtaaaggt gatcgtaaaa gcgatctgcc
     4921 ggttcgtcgt cgtccgcgtc gtagtgccga agaaacccgt cgtgatattc tggcaaaagc
     4981 cgaagaactg tttcgtgaac gtggttttaa tgcagttgcc attgcagata ttgcaagcgc
     5041 actgaatatg agtccggcaa atgtgtttaa acattttagc agcaaaaacg cactggttga
     5101 tgcaattggt tttggtcaga ttggtgtttt tgaacgtcag atttgtccgc tggataaaag
     5161 ccatgcaccg ctggatcgtc tgcgtcatct ggcacgtaat ctgatggaac agcatcatca
     5221 ggatcatttc aaacacatac gggtttttat tcagatcctg atgaccgcca aacaggatat
     5281 gaaatgtggc gattattaca aaagcgtgat tgcaaaactg ctggccgaaa ttattcgtga
     5341 tggtgttgaa gcaggtctgt atattgcaac cgatattccg gttctggcag aaaccgttct
     5401 gcatgcactg accagcgtta ttcatccggt tctgattgca caagaagata ttggtaatct
     5461 ggcaacccgt tgtgatcagc tggttgatct gattgatgca ggtctgcgta atccgctggc
     5521 aaaataacca attattgaac accctaacgg gtgttttttt ttttttggtc tacccgctaa
     5581 cgatcgttgg ctgtgttgac aattaatcat cggctcgtat aatgtgtgga attgtgagcg
     5641 ctcacaattc tgaagtagtc accggctgtg cttgccggtc tgatgagcct gtgaaggcga
     5701 aactacctct acaaataatt ttgtttaaac ccccgagatg aaatacatcc tgtttgaggt
     5761 gtgcgaaatg ggtaaaagcc gtgaacagac catggaaaat attctgaaag cagccaaaaa
     5821 gaaattcggc gaacgtggtt atgaaggcac cagcattcaa gaaattacca aagaagccaa
     5881 agttaacgtt gcaatggcca gctattactt taatggcaaa gagaacctgt actacgaggt
     5941 gttcaaaaaa tacggtctgg caaatgaact gccgaacttt ctggaaaaaa accagtttaa
     6001 tccgattaat gccctgcgtg aatatctgac cgtttttacc acccacatta aagaaaatcc
     6061 ggaaattggc accctggcct atgaagaaat tatcaaagaa agcgcacgcc tggaaaaaat
     6121 caaaccgtat tttatcggca gcttcgaaca gctgaaagaa attctgcaag agggtgaaaa
     6181 acagggtgtg tttcactttt ttagcatcaa ccataccatc cattggatta ccagcattgt
     6241 tctgtttccg aaattcaaaa aattcatcga tagcctgggt ccgaatgaaa ccaatgatac
     6301 caatcatgaa tggatgccgg aagatctggt tagccgtatt attagcgcac tgaccgataa
     6361 accgaacatt taaaacgcat gagaaagccc ccggaagatc accttccggg ggctttttta
     6421 ttgcgcaggt acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca
     6481 tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc
     6541 cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca
     6601 aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt
     6661 gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc
     6721 aactctctac tgtttctcca tacccgtttt tttgggctag cctgaagggt gtctcaaggt
     6781 gcgtaccttg actgatgagt ccgaaaggac gaaacacccc tctacaaata attttgttta
     6841 aaatgttccc taataatcag caaagaggtt actagatggc aggcgcagtt ggtcgtccgc
     6901 gtcgtagtgc accgcgtcgt gcaggtaaaa atccgcgtga agaaattctg gatgcaagcg
     6961 cagaactgtt tacccgtcag ggttttgcaa ccaccagtac ccatcagatt gcagatgcag
     7021 ttggtattcg tcaggcaagc ctgtattatc attttccgag caaaaccgaa atctttctga
     7081 ccctgctgaa aagcaccgtt gaaccgagca ccgttctggc agaagatctg agcaccctgg
     7141 atgcaggtcc ggaaatgcgt ctgtgggcaa ttgttgcaag cgaagttcgt ctgctgctga
     7201 gcaccaaatg gaatgttggt cgtctgtatc agctgccgat tgttggtagc gaagaatttg
     7261 cagaatatca tagccagcgt gaagcactga ccaatgtttt tcgtgatctg gcaaccgaaa
     7321 ttgttggtga tgatccgcgt gcagaactgc cgtttcatat taccatgagc gttattgaaa
     7381 tgcgtcgcaa tgatggtaaa attccgagtc cgctgagcgc agatagcctg ccggaaaccg
     7441 caattatgct ggcagatgca agcctggcag ttctgggtgc accgctgcct gcagatcgtg
     7501 ttgaaaaaac cctggaactg attaaacagg cagatgcaaa ataactcggt accaaagacg
     7561 aacaataaga cgctgaaaag cgtctttttt cgttttggtc caatggtcac catatatcaa
     7621 gtttacggct agctcagtcc taggtactat gctagctact agagaaagag gagaaatact
     7681 agatggctga agcgcaaaat gatcccctgc tgccgggata ctcgtttaat gcccatctgg
     7741 tggcgggttt aacgccgatt gaggccaacg gttatctcga tttttttatc gaccgaccgc
     7801 tgggaatgaa aggttatatt ctcaatctca ccattcgcgg tcagggggtg gtgaaaaatc
     7861 agggacgaga atttgtttgc cgaccgggtg atattttgct gttcccgcca ggagagattc
     7921 atcactacgg tcgtcatccg gaggctcgcg aatggtatca ccagtgggtt tactttcgtc
     7981 cgcgcgccta ctggcatgaa tggcttaact ggccgtcaat atttgccaat acggggttct
     8041 ttcgcccgga tgaagcgcac cagccgcatt tcagcgacct gtttgggcaa atcattaacg
     8101 ccgggcaagg ggaagggcgc tattcggagc tgctggcgat aaatctgctt gagcaattgt
     8161 tactgcggcg catggaagcg attaacgagt cgctccatcc accgatggat aatcgggtac
     8221 gcgaggcttg tcagtacatc agcgatcacc tggcagacag caattttgat atcgccagcg
     8281 tcgcacagca tgtttgcttg tcgccgtcgc gtctgtcaca tcttttccgc cagcagttag
     8341 ggattagcgt cttaagctgg cgcgaggacc aacgtatcag ccaggcgaag ctgcttttga
     8401 gcaccacccg gatgcctatc gccaccgtcg gtcgcaatgt tggttttgac gatcaactct
     8461 atttctcgcg ggtatttaaa aaatgcaccg gggccagccc gagcgagttc cgtgccggtt
     8521 aataaccaat tattgaaggc cgctaacgca gccttttttt gtttctggtc tcccaatggc
     8581 ggcgcgccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     8641 gtcaattcag ggtggtgaat atgaaaccag taacgttata cgatgtcgca gagtatgccg
     8701 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     8761 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     8821 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     8881 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     8941 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     9001 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     9061 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     9121 cacccatcaa cagtattatt ttctcccatg aggacggtac gcgactgggc gtggagcatc
     9181 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     9241 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     9301 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     9361 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     9421 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
     9481 acgataccga agatagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     9541 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     9601 agggcaatca gctgttgcca gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     9661 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     9721 cccgactgga aagcgggcag tgataatcca ggaggaaaaa aatgtccaga ttagataaaa
     9781 gtaaagtgat taacagcgca ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa
     9841 cccgtaaact cgcccagaag ctaggtgtag agcagcctac attgtattgg catgtaaaaa
     9901 ataagcgggc tttgctcgac gccttagcca ttgagatgtt agataggcac catactcact
     9961 tttgcccttt agaaggggaa agctggcaag attttttacg taataacgct aaaagtttta
    10021 gatgtgcttt actaagtcat cgcgatggag caaaagtaca tttaggtaca cggcctacag
    10081 aaaaacagta tgaaactctc gaaaatcaat tagccttttt atgccaacaa ggtttttcac
    10141 tagagaatgc attatatgca ctcagcgctg tggggcattt tactttaggt tgcgtattgg
    10201 aagatcaaga gcatcaagtc gctaaagaag aaagggaaac acctactact gatagtatgc
    10261 cgccattatt acgacaagct atcgaattat ttgatcacca aggtgcagag ccagccttct
    10321 tattcggcct tgaattgatc atatgcggat tagaaaaaca acttaaatgt gaaagtgggt
    10381 cctaataatt ggtaacgaat cagacaattg acggctcgag ggagtagcat agggtttgca
    10441 gaatccctgc ttcgtccatt tgacaggcac attatgcatc gatgataagc tgtcaaacat
    10501 gagcagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
    10561 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
    10621 atgagcaaat attttatctg aggt
//
