LOCUS       sequence-147455-        8653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Human expression plasmid for LbCpf1-NLS-3xHA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Kleinstiver BP, Tsai SQ, Prew MS, Nguyen NT, Welch MM, Lopez JM, 
            McCaw ZR, Aryee MJ, Joung JK
  TITLE     Genome-wide specificities of CRISPR-Cas Cpf1 nucleases in human 
            cells.
  JOURNAL   Nat Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620.
  PUBMED    27347757
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2016 Jun 27. doi: 10.1038/nbt.3620."
COMMENT     Sequence Label: SQT1665
FEATURES             Location/Qualifiers
     source          1..8653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1769..5452
                     /codon_start=1
                     /product="CRISPR-associated protein from Lachnospiraceae 
                     bacterium ND2006 (Zetsche et al., 2015)"
                     /label=LbCpf1
                     /translation="MSKLEKFTNCYSLSKTLRFKAIPVGKTQENIDNKRLLVEDEKRAE
                     DYKGVKKLLDRYYLSFINDVLHSIKLKNLNNYISLFRKKTRTEKENKELENLEINLRKE
                     IAKAFKGNEGYKSLFKKDIIETILPEFLDDKDEIALVNSFNGFTTAFTGFFDNRENMFS
                     EEAKSTSIAFRCINENLTRYISNMDIFEKVDAIFDKHEVQEIKEKILNSDYDVEDFFEG
                     EFFNFVLTQEGIDVYNAIIGGFVTESGEKIKGLNEYINLYNQKTKQKLPKFKPLYKQVL
                     SDRESLSFYGEGYTSDEEVLEVFRNTLNKNSEIFSSIKKLEKLFKNFDEYSSAGIFVKN
                     GPAISTISKDIFGEWNVIRDKWNAEYDDIHLKKKAVVTEKYEDDRRKSFKKIGSFSLEQ
                     LQEYADADLSVVEKLKEIIIQKVDEIYKVYGSSEKLFDADFVLEKSLKKNDAVVAIMKD
                     LLDSVKSFENYIKAFFGEGKETNRDESFYGDFVLAYDILLKVDHIYDAIRNYVTQKPYS
                     KDKFKLYFQNPQFMGGWDKDKETDYRATILRYGSKYYLAIMDKKYAKCLQKIDKDDVNG
                     NYEKINYKLLPGPNKMLPKVFFSKKWMAYYNPSEDIQKIYKNGTFKKGDMFNLNDCHKL
                     IDFFKDSISRYPKWSNAYDFNFSETEKYKDIAGFYREVEEQGYKVSFESASKKEVDKLV
                     EEGKLYMFQIYNKDFSDKSHGTPNLHTMYFKLLFDENNHGQIRLSGGAELFMRRASLKK
                     EELVVHPANSPIANKNPDNPKKTTTLSYDVYKDKRFSEDQYELHIPIAINKCPKNIFKI
                     NTEVRVLLKHDDNPYVIGIDRGERNLLYIVVVDGKGNIVEQYSLNEIINNFNGIRIKTD
                     YHSLLDKKEKERFEARQNWTSIENIKELKAGYISQVVHKICELVEKYDAVIALEDLNSG
                     FKNSRVKVEKQVYQKFEKMLIDKLNYMVDKKSNPCATGGALKGYQITNKFESFKSMSTQ
                     NGFIFYIPAWLTSKIDPSTGFVNLLKTKYTSIADSKKFISSFDRIMYVPEEDLFEFALD
                     YKNFSRTDADYIKKWKLYSYGNRIRIFRNPKKNNVFDWEEVCLTSAYKELFNKYGINYQ
                     QGDIRALLCEQSDKAFYSSFMALMSLMLQMRNSITGRTDVDFLISPVKNSDGIFYDSRN
                     YEAQENAILPKNADANGAYNIARKVLWAIGQFKKAEDEKLDKVKIAISNKEWLEYAQTS
                     VKH"
     CDS             5453..5500
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5507..5587
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5677..5696)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5742..5797
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5796..5815)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6156..6172)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6156..6172)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6169..6191)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6180..6196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6204..6210,6211..6228,6229..6234))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6249..6270
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6329..6524
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6375..6510
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6530..6664
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6567..6586)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6621..6640
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6732..6749)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6903..7491)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6983..7002)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7662..8453,8454..8522))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8285..8304
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8523..8627)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attctgcagt cgacggtacc
     1741 gcgggcccgg gatccaccgg tcgccaccat gagcaagctg gagaagttta caaactgcta
     1801 ctccctgtct aagaccctga ggttcaaggc catccctgtg ggcaagaccc aggagaacat
     1861 cgacaataag cggctgctgg tggaggacga gaagagagcc gaggattata agggcgtgaa
     1921 gaagctgctg gatcgctact atctgtcttt tatcaacgac gtgctgcaca gcatcaagct
     1981 gaagaatctg aacaattaca tcagcctgtt ccggaagaaa accagaaccg agaaggagaa
     2041 taaggagctg gagaacctgg agatcaatct gcggaaggag atcgccaagg ccttcaaggg
     2101 caacgagggc tacaagtccc tgtttaagaa ggatatcatc gagacaatcc tgccagagtt
     2161 cctggacgat aaggacgaga tcgccctggt gaacagcttc aatggcttta ccacagcctt
     2221 caccggcttc tttgataaca gagagaatat gttttccgag gaggccaaga gcacatccat
     2281 cgccttcagg tgtatcaacg agaatctgac ccgctacatc tctaatatgg acatcttcga
     2341 gaaggtggac gccatctttg ataagcacga ggtgcaggag atcaaggaga agatcctgaa
     2401 cagcgactat gatgtggagg atttctttga gggcgagttc tttaactttg tgctgacaca
     2461 ggagggcatc gacgtgtata acgccatcat cggcggcttc gtgaccgaga gcggcgagaa
     2521 gatcaagggc ctgaacgagt acatcaacct gtataatcag aaaaccaagc agaagctgcc
     2581 taagtttaag ccactgtata agcaggtgct gagcgatcgg gagtctctga gcttctacgg
     2641 cgagggctat acatccgatg aggaggtgct ggaggtgttt agaaacaccc tgaacaagaa
     2701 cagcgagatc ttcagctcca tcaagaagct ggagaagctg ttcaagaatt ttgacgagta
     2761 ctctagcgcc ggcatctttg tgaagaacgg ccccgccatc agcacaatct ccaaggatat
     2821 cttcggcgag tggaacgtga tccgggacaa gtggaatgcc gagtatgacg atatccacct
     2881 gaagaagaag gccgtggtga ccgagaagta cgaggacgat cggagaaagt ccttcaagaa
     2941 gatcggctcc ttttctctgg agcagctgca ggagtacgcc gacgccgatc tgtctgtggt
     3001 ggagaagctg aaggagatca tcatccagaa ggtggatgag atctacaagg tgtatggctc
     3061 ctctgagaag ctgttcgacg ccgattttgt gctggagaag agcctgaaga agaacgacgc
     3121 cgtggtggcc atcatgaagg acctgctgga ttctgtgaag agcttcgaga attacatcaa
     3181 ggccttcttt ggcgagggca aggagacaaa cagggacgag tccttctatg gcgattttgt
     3241 gctggcctac gacatcctgc tgaaggtgga ccacatctac gatgccatcc gcaattatgt
     3301 gacccagaag ccctactcta aggataagtt caagctgtat tttcagaacc ctcagttcat
     3361 gggcggctgg gacaaggata aggagacaga ctatcgggcc accatcctga gatacggctc
     3421 caagtactat ctggccatca tggataagaa gtacgccaag tgcctgcaga agatcgacaa
     3481 ggacgatgtg aacggcaatt acgagaagat caactataag ctgctgcccg gccctaataa
     3541 gatgctgcca aaggtgttct tttctaagaa gtggatggcc tactataacc ccagcgagga
     3601 catccagaag atctacaaga atggcacatt caagaagggc gatatgttta acctgaatga
     3661 ctgtcacaag ctgatcgact tctttaagga tagcatctcc cggtatccaa agtggtccaa
     3721 tgcctacgat ttcaactttt ctgagacaga gaagtataag gacatcgccg gcttttacag
     3781 agaggtggag gagcagggct ataaggtgag cttcgagtct gccagcaaga aggaggtgga
     3841 taagctggtg gaggagggca agctgtatat gttccagatc tataacaagg acttttccga
     3901 taagtctcac ggcacaccca atctgcacac catgtacttc aagctgctgt ttgacgagaa
     3961 caatcacgga cagatcaggc tgagcggagg agcagagctg ttcatgaggc gcgcctccct
     4021 gaagaaggag gagctggtgg tgcacccagc caactcccct atcgccaaca agaatccaga
     4081 taatcccaag aaaaccacaa ccctgtccta cgacgtgtat aaggataaga ggttttctga
     4141 ggaccagtac gagctgcaca tcccaatcgc catcaataag tgccccaaga acatcttcaa
     4201 gatcaataca gaggtgcgcg tgctgctgaa gcacgacgat aacccctatg tgatcggcat
     4261 cgataggggc gagcgcaatc tgctgtatat cgtggtggtg gacggcaagg gcaacatcgt
     4321 ggagcagtat tccctgaacg agatcatcaa caacttcaac ggcatcagga tcaagacaga
     4381 ttaccactct ctgctggaca agaaggagaa ggagaggttc gaggcccgcc agaactggac
     4441 ctccatcgag aatatcaagg agctgaaggc cggctatatc tctcaggtgg tgcacaagat
     4501 ctgcgagctg gtggagaagt acgatgccgt gatcgccctg gaggacctga actctggctt
     4561 taagaatagc cgcgtgaagg tggagaagca ggtgtatcag aagttcgaga agatgctgat
     4621 cgataagctg aactacatgg tggacaagaa gtctaatcct tgtgcaacag gcggcgccct
     4681 gaagggctat cagatcacca ataagttcga gagctttaag tccatgtcta cccagaacgg
     4741 cttcatcttt tacatccctg cctggctgac atccaagatc gatccatcta ccggctttgt
     4801 gaacctgctg aaaaccaagt ataccagcat cgccgattcc aagaagttca tcagctcctt
     4861 tgacaggatc atgtacgtgc ccgaggagga tctgttcgag tttgccctgg actataagaa
     4921 cttctctcgc acagacgccg attacatcaa gaagtggaag ctgtactcct acggcaaccg
     4981 gatcagaatc ttccggaatc ctaagaagaa caacgtgttc gactgggagg aggtgtgcct
     5041 gaccagcgcc tataaggagc tgttcaacaa gtacggcatc aattatcagc agggcgatat
     5101 cagagccctg ctgtgcgagc agtccgacaa ggccttctac tctagcttta tggccctgat
     5161 gagcctgatg ctgcagatgc ggaacagcat cacaggccgc accgacgtgg attttctgat
     5221 cagccctgtg aagaactccg acggcatctt ctacgatagc cggaactatg aggcccagga
     5281 gaatgccatc ctgccaaaga acgccgacgc caatggcgcc tataacatcg ccagaaaggt
     5341 gctgtgggcc atcggccagt tcaagaaggc cgaggacgag aagctggata aggtgaagat
     5401 cgccatctct aacaaggagt ggctggagta cgcccagacc agcgtgaagc acaaaaggcc
     5461 ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag ggatcctacc catacgatgt
     5521 tccagattac gcttatccct acgacgtgcc tgattatgca tacccatatg atgtccccga
     5581 ctatgcctaa agcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     5641 tgtggccaat gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     5701 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     5761 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     5821 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg
     5881 ctggctgcca tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg
     5941 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
     6001 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
     6061 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat
     6121 ccctcgacct gcagcccaag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6181 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6241 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6301 tcgggaaacc tgtcgtgcca gcggatccgc atctcaatta gtcagcaacc atagtcccgc
     6361 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     6421 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     6481 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagcta acttgtttat
     6541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     6601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     6661 gatccgctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     6721 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     6781 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     6841 aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     6901 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     6961 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     7021 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     7081 agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     7141 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     7201 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     7261 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     7321 cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt
     7381 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     7441 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     7501 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     7561 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     7621 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
     7681 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     7741 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     7801 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     7861 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     7921 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     7981 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     8041 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     8101 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     8161 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     8221 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     8281 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     8341 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     8401 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     8461 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     8521 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     8581 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     8641 aaagtgccac ctg
//
