LOCUS       sequence-147463-         940 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shETV5 shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pLKO.5 shETV5-A
FEATURES             Location/Qualifiers
     source          1..940
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(22..505)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(58..76)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    554..671
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     complement(826..845)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 agagaggtcg gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt
       61 gcagaatgcg gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc
      121 ctcccgcacc gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt
      181 ggccacagcc catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag
      241 ggtactagtg agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga
      301 accttcccga cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc
      361 gaagatccgg gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc
      421 gctgcgtttc ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc
      481 ttggaaaagg cgcaacccgg gccccctcga gccggcgcca aagtggatct ctgctgtccc
      541 tgtaataaac ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta
      601 tgtctgttgc tattatgtct actattcttt cccctgcact gtacccccca atcccccctt
      661 ttcttttaaa attgtggatg aatactgcca tttgtctcaa gatctagaat tcaaaaacac
      721 ctccaaccaa gatcaaacct cgaggtttga tcttggttgg aggtgccggt acctcgtcct
      781 ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt aagcatatga
      841 tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta ttactttcta
      901 cgtcacgtat tttgtactaa tatctttgtg tttacagtca
//
