LOCUS       sequence-147466-        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses sfCherry(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pcDNA3.1-sfCherry(1-10)
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(833..850)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    839..950
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccatggagga
      121 ggacaacatg gccatcatca aggagttcat gagattcaag gtgcacatgg agggcagcgt
      181 gaacggccac gagttcgaga tcgagggcga gggcgagggc cacccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc ttcgcctggg acatcctgag
      301 cccccagttc atgtacggca gcaaggccta cgtgaagcac cccgccgaca tccccgacta
      361 cctgaagctg agcttccccg agggcttcac ctgggagaga gtgatgaact tcgaggacgg
      421 cggcgtggtg accgtgaccc aggacagcag cctgcaggac ggcgagttca tctacaaggt
      481 gaagctgctg ggcaccaact tccccagcga cggccccgtg atgcagaaga agaccatggg
      541 ctgggaggcc agcaccgaga gaatgtaccc cgaggacggc gccctgaagg gcgagatcaa
      601 ccagagactg aagctgaagg acggcggcca ctacgacgcc gaggtgaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtg gacatcaagc tggacatcac
      721 cagccacaac gaggactgag gatccactag tccagtgtgg tggaattctg cagatatcca
      781 gcacagtggc ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact
      841 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      901 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      961 agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg g
//
