LOCUS       sequence-147475-         861 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation lentiviral vector encoding human p53 with a 
            C-terminal V5 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 861)
  AUTHORS   Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW
  TITLE     Different mutant/wild-type p53 combinations cause a spectrum of 
            increased invasive potential in nonmalignant immortalized human 
            mammary epithelial cells.
  JOURNAL   Neoplasia. 2008 May . 10(5):450-61.
  PUBMED    18472962
REFERENCE   2  (bases 1 to 861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neoplasia. 
            2008 May . 10(5):450-61."
COMMENT     Sequence Label: pLenti6/V5-p53_wt p53
FEATURES             Location/Qualifiers
     source          1..861
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..48)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ggtacgcgta gaatcgagac cgaggagagg gttagggata ggcttacctt cgaaccgcgg
       61 gccctctaga ctcgagccct tgtctgagtc aggcccttct gtcttgaaca tgagtttttt
      121 atggcgggag gtagactgac cctttttgga cttcaggtgg ctggagtgag ccctgctccc
      181 ccctggctcc ttcccagcct gggcatcctt gagttccaag gcctcattca gctctcggaa
      241 catctcgaag cgctcacgcc cacggatctg aagggtgaaa tattctccat ccagtggttt
      301 cttctttggc tggggagagg agctggtgtt gttgggcagt gctcgcttag tgctccctgg
      361 gggcagctcg tggtgaggct cccctttctt gcggagattc tcttcctctg tgcgccggtc
      421 tctcccagga caggcacaaa cacgcacctc aaagctgttc cgtcccagta gattaccact
      481 ggagtcttcc agtgtgatga tggtgaggat gggcctccgg ttcatgccgc ccatgcagga
      541 actgttacac atgtagttgt agtggatggt ggtacagtca gagccaacct caggcggctc
      601 atagggcacc accacactat gtcgaaaagt gtttctgtca tccaaatact ccacacgcaa
      661 atttccttcc actcggataa gatgctgagg aggggccaga ccatcgctat ctgagcagcg
      721 ctcatggtgg gggcagcgcc tcacaacctc cgtcatgtgc tgtgactgct tgtagatggc
      781 catggcgcgg acgcgggtgc cgggcggggg tgtggaatca acccacagct gcacagggca
      841 ggtcttggcc agttggcaaa a
//
