LOCUS       sequence-147480-        2307 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which 
            contains eSpCas9 and puromycin cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2307)
  AUTHORS   Sikder RK, Ellithi M, Uzzo RN, Weader D, Metz AL, Behbahani A, 
            McKenzie ER, El-Deiry WS, Abbosh PH
  TITLE     Differential effects of clinically-relevant N- vs C-terminal 
            truncating CDKN1A mutations on cisplatin sensitivity in bladder 
            cancer.
  JOURNAL   Mol Cancer Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200.
  PUBMED    33272936
REFERENCE   2  (bases 1 to 2307)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2020 Dec 3. pii: 1541-7786.MCR-19-1200. doi: 
            10.1158/1541-7786.MCR-19-1200."
COMMENT     Sequence Label: pLentiCRISPR-E
FEATURES             Location/Qualifiers
     source          1..2307
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2254..2301
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 gatatcgtgc tgaccctgac actgtttgag gacagagaga tgatcgagga acggctgaaa
       61 acctatgccc acctgttcga cgacaaagtg atgaagcagc tgaagcggcg gagatacacc
      121 ggctggggca ggctgagccg gaagctgatc aacggcatcc gggacaagca gtccggcaag
      181 acaatcctgg atttcctgaa gtccgacggc ttcgccaaca gaaacttcat gcagctgatc
      241 cacgacgaca gcctgacctt taaagaggac atccagaaag cccaggtgtc cggccagggc
      301 gatagcctgc acgagcacat tgccaatctg gccggcagcc ccgccattaa gaagggcatc
      361 ctgcagacag tgaaggtggt ggacgagctc gtgaaagtga tgggccggca caagcccgag
      421 aacatcgtga tcgaaatggc cagagagaac cagaccaccc agaagggaca gaagaacagc
      481 cgcgagagaa tgaagcggat cgaagagggc atcaaagagc tgggcagcca gatcctgaaa
      541 gaacaccccg tggaaaacac ccagctgcag aacgagaagc tgtacctgta ctacctgcag
      601 aatgggcggg atatgtacgt ggaccaggaa ctggacatca accggctgtc cgactacgat
      661 gtggaccata tcgtgcctca gagctttctg gccgacgact ccatcgacaa caaggtgctg
      721 accagaagcg acaagaaccg gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag
      781 aagatgaaga actactggcg gcagctgctg aacgccaagc tgattaccca gagaaagttc
      841 gacaatctga ccaaggccga gagaggcggc ctgagcgaac tggataaggc cggcttcatc
      901 aagagacagc tggtggaaac ccggcagatc acaaagcacg tggcacagat cctggactcc
      961 cggatgaaca ctaagtacga cgagaatgac aagctgatcc gggaagtgaa agtgatcacc
     1021 ctgaagtcca agctggtgtc cgatttccgg aaggatttcc agttttacaa agtgcgcgag
     1081 atcaacaact accaccacgc ccacgacgcc tacctgaacg ccgtcgtggg aaccgccctg
     1141 atcaaaaagt accctgcgct ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac
     1201 gtgcggaaga tgatcgccaa gagcgagcag gaaatcggca aggctaccgc caagtacttc
     1261 ttctacagca acatcatgaa ctttttcaag accgagatta ccctggccaa cggcgagatc
     1321 cggaaggcgc ctctgatcga gacaaacggc gaaaccgggg agatcgtgtg ggataagggc
     1381 cgggattttg ccaccgtgcg gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag
     1441 accgaggtgc agacaggcgg cttcagcaaa gagtctatcc tgcccaagag gaacagcgat
     1501 aagctgatcg ccagaaagaa ggactgggac cctaagaagt acggcggctt cgacagcccc
     1561 accgtggcct attctgtgct ggtggtggcc aaagtggaaa agggcaagtc caagaaactg
     1621 aagagtgtga aagagctgct ggggatcacc atcatggaaa gaagcagctt cgagaagaat
     1681 cccatcgact ttctggaagc caagggctac aaagaagtga aaaaggacct gatcatcaag
     1741 ctgcctaagt actccctgtt cgagctggaa aacggccgga agagaatgct ggcctctgcc
     1801 ggcgaactgc agaagggaaa cgaactggcc ctgccctcca aatatgtgaa cttcctgtac
     1861 ctggccagcc actatgagaa gctgaagggc tcccccgagg ataatgagca gaaacagctg
     1921 tttgtggaac agcacaagca ctacctggac gagatcatcg agcagatcag cgagttctcc
     1981 aagagagtga tcctggccga cgctaatctg gacaaagtgc tgtccgccta caacaagcac
     2041 cgggataagc ccatcagaga gcaggccgag aatatcatcc acctgtttac cctgaccaat
     2101 ctgggagccc ctgccgcctt caagtacttt gacaccacca tcgaccggaa gaggtacacc
     2161 agcaccaaag aggtgctgga cgccaccctg atccaccaga gcatcaccgg cctgtacgag
     2221 acacggatcg acctgtctca gctgggaggc gacaaaaggc cggcggccac gaaaaaggcc
     2281 ggccaggcaa aaaagaaaaa gggatcc
//
