LOCUS       sequence-147483-       12586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GESTALT V6 barcode for zebrafish.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12586)
  AUTHORS   McKenna A, Findlay GM, Gagnon JA, Horwitz MS, Schier AF, Shendure J
  TITLE     Whole organism lineage tracing by combinatorial and cumulative 
            genome editing.
  JOURNAL   Science. 2016 May 26. pii: aaf7907.
  PUBMED    27229144
REFERENCE   2  (bases 1 to 12586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 May 26. pii: aaf7907."
COMMENT     Sequence Label: pTol2-DRv6
FEATURES             Location/Qualifiers
     source          1..12586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(36..464)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(146..167)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     358..377
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     591..613
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     605..622
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     606..622
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        632..650
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(2536..2547,2548..2710)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     regulatory      6636..6645
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6642..7319
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSENVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(6772..6790)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(6822..6842)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     7040..7059
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     7234..7257
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    7662..7796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(8098..8811,8812..8814,8815..8817))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(8101..8811,8812..8814,8815..8817))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8143..8164)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8492..8511
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8751..8772
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(10116..10303,10304..10315)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10349..10365)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10349..10365)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10402..10422)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10402..10420)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10441..10457)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10441..10457)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10454..10476)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10465..10481
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10489..10495,10496..10513,10514..10519))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10534..10555
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10672..10689)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10843..11431)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10923..10942)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11602..12393,12394..12462))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12225..12244
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12463..12567)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
       61 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
      121 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
      181 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
      241 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
      301 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
      361 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
      421 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag
      481 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
      541 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
      601 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg
      661 taccaaatag taggaattac ccacctgtac aagtgctgaa aacttggatg aataagcccg
      721 tttgccattt tctactgcta ttttaaatct tttctgtatt tgtctgcatt tgtctttacc
      781 cttgaataaa tgttttagtt gttttttttc aattatgact gtgtttaaca gacattatta
      841 caataatatg taatagtagt acacactatt attatgtaat agtacttgtt gactgtattt
      901 gagaactgga ccgagtgagt gttacgtcac ccattcaaaa tgacttactt ctggctccaa
      961 caaaatgaag ttaattcagt tgccattttt cactgtatgg acatcgccgt gttggagcta
     1021 gacgtcgcca ataagcaaga aagagccgag atgcgtcgag tctgagtcac cgttcctatg
     1081 gcaacccctc taaccaatca gaagtaagct tgttggaagt ccacagccta ccacttgaaa
     1141 gcgggctgca caaaatctgt caaacgtttt gaacgttgga tgtgagagca catactttta
     1201 ttaaggcatc tggttggtca gtttataata tcaacaactt gggctacaga aaagaaaagt
     1261 tattacagaa attatgatta acaagtacat gttaaataaa gattttaata tgaatgccac
     1321 cactggagca ttcatgccat ttggagcttc ttcctgtttg gatcactaga aggaggaggt
     1381 cactcattac agttctcata tacagtcgtt ggttggttgg ttggttggtt ggttggtaga
     1441 ttgattgatt gattgattga ttgattgatt gattgattga ttgattgatt gattgattga
     1501 ttgattgatt gattgattga ttgattgatt gattgattga ttggtagtca aaataagaaa
     1561 taatttccac agattcatta cagaaatgat taaatgcata cataaaaaac tggggggggg
     1621 gggatacaac aacacactta agtcacattt gcctacgtaa agaaaagtaa agaaaatcaa
     1681 tagctatatt ttacatctcc tttttttgct gtcttttaat tagccttgtt ttgctgttat
     1741 cttgattgaa actgtaactt cttcacctgc ttcttttctt tgtaggtttt gccagcccag
     1801 aaacaggcat ggctgtgcaa ggccagagca ccatgcacaa tgcgctgcat gtcttcatga
     1861 acggctcaat gtcctcagtc cagggctcag ccaacgaccc catcttcctc cttcaccatg
     1921 ctttcattga caggtaacaa acacgtcatg acattagact gcacagtttt tgacaaagtt
     1981 catacaatct gttgtttata gctgctacaa ttagtgaagt ttgtgaatgt acttggatga
     2041 gcagcgaaag atcaattgag atcaattgtt agagtttggt tgccctgcag agcaaagaac
     2101 aaaaaataat ctggtggctt tactgcgtga ggttattatt ggtggaatag aaacacaaaa
     2161 cataattgca tttatttgtt taatttttta tcttatctta actttcatct tgcatatttg
     2221 tttcttacat catttctagc atctttgagc gctggctaag aactcatcag cctccccggt
     2281 ccatctaccc acgtaccaat gcaccaattg gccacaatga cggctactac atggtgccat
     2341 tccttcctct ttataggaat ggagactacc tcctgtccaa caatgctctt ggatacgagt
     2401 acgcctacct gttggaccca ggtcattgca caacaccaga aatgccctct gatctgcaaa
     2461 agacgtgaat atctgttcag acacccatat gcactctgtt ccacacaggt cagaggtttg
     2521 tccaggagtt cttgacagag gtgtaaaaag tactcaaaaa ttttactcaa gtgaaagtac
     2581 aagtacttag ggaaaatttt actcaattaa aagtaaaagt atctggctag aatcttactt
     2641 gagtaaaagt aaaaaagtac tccattaaaa ttgtacttga gtattaagga agtaaaagta
     2701 aaagcaagaa agaaaactag agattcttgt ttaagctttt aatctcaaaa aacattaaat
     2761 gaaatgcata caaggtttta tcctgcttta gaactgtttg tatttaatta tcaaactata
     2821 agacagacaa tctaatgcca gtacacgcta ctcaaagttg taaaacctca gatttaactt
     2881 cagtagaagc tgattctcaa aattgttagt gtcaagccta gctcttttgg ggctgaaaag
     2941 caatcctgca gtgctgaaaa gcctctcaca ggcagccgat gcgggaagag gtgtattagt
     3001 cttgatagag aggctgcaaa tagcaggaaa cgtgagcaga gactccctgg tgtctgaaac
     3061 acaggccaga tgggccattc gtacgcttaa tttcgtatag ggataacagg gtaatgatct
     3121 aggtcgacac cagcaaagtt ctagaatttg tcgaaacatt tatgttatat atttcctgaa
     3181 aaaaattctg agtaagttct taagtgttat tgccagcaac ataaacaaca gacggcaaaa
     3241 tgaataaatg ataacaaagc agtaggctta aataaaccta atttttatag gctgttctct
     3301 acaaccctca aacagtgatt agttttgtac ttataaactt gccctttcat tcatatttca
     3361 agaaaattgg ttcagaagat ctggatattc tagcagttgt tcaagctcat ggagggatca
     3421 gtgacctgat tccacaatga ctaggcctaa tccagaaatt agatgactgt caacataaaa
     3481 aggcacagca ctcactagct gccctatata ttttattata ttttacatat attattttat
     3541 ttatttagct ctgagtgctg tactttctgg ttaaagaaaa ctgcttacaa cagctaacct
     3601 gtactacctc aggctcaggg aatttggaac aggtttgtct ggtttgtttc tttaaccatg
     3661 catgcttgtt ttcaactatg gcaacacagt cacatgggac attacagaaa tgatttgtcg
     3721 atgacatgcg acttttcttt aataaagcgc aaagatccca aaaagcaaac ttttaacaaa
     3781 aatcatataa ttatattttc aatccagctt tgtagcaact ttgtgctgct gttcactcag
     3841 caacagatag tcagtataag gtcagtgtgt ctcaaagcag tgccatctgt ttcacacatt
     3901 gcgttctata tataagtgtg ctggttgaca cgacactgta taaggcctag gctaaaacac
     3961 aaacaatgta gaatgacact gtgttttttt tgtaaacaaa tgttgttttt ggttaaacat
     4021 ctttgtgaaa acatcctcct gtcatgtatt tgctatattc aaatgttaaa cccgtgcaga
     4081 atagaacata tacaaaaaaa aacaacacaa cacattttta aacattatta aatatcaagt
     4141 attgctggca gttctgtttc tgttttacag taccctttgc cacagttctc cgcttttcct
     4201 ggtccagatt ccacaagtct gattcaccaa tagcaaagcg aataaacaac caaagcagcc
     4261 aatcactgct tgtagactgt cctgcgagac cggcccattc cagcacattc tggaaacttc
     4321 ctttatatga taattataaa tacatttaaa ttattgatac aaaacatgta attcctagaa
     4381 cataaccata gcaatcatta gttttcaggg taattatgta tttttaggat ttgactgcgg
     4441 aaagatctgg tcatgtgacg tctcatgaac gtcacggccc tgggtttcta taaatacagt
     4501 aggactctcg accatcggca gatttttcga agaagaagat cagtttcagg agccgtactg
     4561 ttccgtttca acgcaaatat taacggtaag agcgaatttc ctagtttgtt ttcatgccat
     4621 tctttaaaac catagcgtat tactttaatt atagtaaact ttcgctttct ttattacaag
     4681 agacgttttg tgttgattct ccgcggacat tttcggtcag acaatcagaa aatgaccgcg
     4741 gaggaccagt aacttgcatt acacgtaagt taaatcttcg tgtattaaaa tggttaggtt
     4801 gttaacgtca aataggttac cgtgtttgcg tgtgatcagg ttggttttgt tagatttttg
     4861 tcagtatttt taatttattt gttttagttt atttattttt tttgctgaat catagtttgt
     4921 gaacaaagaa cccggatgtt acatacagta cagccgccat gttacagaga gttataactt
     4981 aatcatttta aaaataattt tgccttactt ttagtttgtc atgttgagaa atgaggaaat
     5041 gttaaaatga ggaaatatcc aattaattta atatatcaaa ataatccatg attacaatgc
     5101 actgaactgg agaaaattaa gatgttttct agtgtcatga aacaaatgta agagatgtac
     5161 attgtagatg ttttatgtca agaattggct agttgatgca gcatactggc gatactcagt
     5221 tgtaataaca gtaacgttac atgttaatag actactgagt atgctgttct gtctatgtat
     5281 gctctgtaag ctacgagaag gactttttta aacagtaaag ggtgcaatat ttttacaaat
     5341 tgaattaaat aaaggctgtc tattaagtaa tatgcttgat atttttctta cttgatcgga
     5401 aataagaaaa aatataaacg ttgttgctct aaaaatccta gttcagttta gccaaccaca
     5461 aatacctttt tgttcctcca acagtttttt tttcttctct ataatatttg gcagtctata
     5521 gtactccaaa tgtttcccca cagtctaact aattggtaca gccaaaatca tgacacttat
     5581 tgcaataata attttggttc attggcattg ttgatagcct gtgccactaa tatggtcgat
     5641 tgatcatgct tcaggaagaa aactatattg tttgatgtaa gattattaaa tcttcacctg
     5701 cttccattac aaactattcc catcttattg aattctggta tgtcttaaag gattagttca
     5761 cttcccaaat caaaatttac ttagcctttt ttcatccatg atcccttttt ttcatcatta
     5821 atgaagaaat tgtttttgaa aaagtttcaa gatttttttc tctatattgt ggagcttgtt
     5881 agtttaaaat tccaaaatgc aatatgtggc ttcaaatggt tctaaatgat cccagtcaag
     5941 gaataacagt cttatctaat gaaaccatta gacctttaaa aataaaaaaa ataaaagtat
     6001 ttatttttaa atgactgagt gattaagttg aatttcagcg tttccttact gtgtagaagt
     6061 ccttccttac tggccccacc ctttggttct ctgccaatct gctacctaat gtaatgttgt
     6121 ggaacattat tattctttat ttcttaattt tttatttttt attttaaaac aatgtaaact
     6181 gcacagatgt gcagtttgtt taaaatggcc aatgctttgg aaatgcatga cataattaga
     6241 tttcatgatg cacaaagcca aatctcagag cttgtgcaaa atgagctatc atttcactag
     6301 gtaagaccct aaattttcat ataggatcat ttggacaatt ttgctgcagg taaaatgcat
     6361 tctatagtcc actgtcagcc attgttttgg atagtattta tttttctcta caagtatagt
     6421 caatagtttt ctattatttt aaaggtttgt aacatttaag ggtgaccaaa tgcaaagtaa
     6481 aatttcattt tcgggtgaac tatctcgttt aacatgggag aagtgcaaaa catacattat
     6541 tggctagaac attgtagtat tttttaaatg gaaatgtgtg attgctaatc ttactttgaa
     6601 tttgtttaca gggatccaaa ctcgagaaag ctagcgccac catggcctcc tccgagaacg
     6661 tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg ctccgtgaac ggccacgagt
     6721 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     6781 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttccagt
     6841 acggctccaa ggtgtacgtg aagcaccccg ccgacatccc cgactacaag aagctgtcct
     6901 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg
     6961 tgacccagga ctcctccctg caggacggct gcttcatcta caaggtgaag ttcatcggcg
     7021 tgaacttccc ctccgacggc cccgtaatgc agaagaagac tatgggctgg gagccctcca
     7081 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccacaag gccctgaagc
     7141 tgaaggacgg cggccactac ctggtggagt tcaagtccat ctacatggcc aagaagcccg
     7201 tgcagctgcc cggctactac tacgtggact ccaagctgga catcacctcc cacaacgagg
     7261 actacaccat cgtggagcag tacgagcgcg ccgagggccg ccaccacctg ttcctgtaga
     7321 aatccggact cagatctcga gctcaagctt cggatacgat acgcgcacgc tatggagtcg
     7381 acacgactcg cgcatacgat ggagtcgata gtatgcgtat acgctatgga gtcgatatgc
     7441 atagcgcatg ctatggagtc gagtcgagac gctgacgata tggagtcgct acgatacact
     7501 ctgactatgg agtcgcgact gtacgcacac gcgatggagt cgatacgtag cacgcagact
     7561 atggagtcga cacagtactc tcactctatg gagtcgatat gagactcgca tgtgatggga
     7621 attctcgacc tcgagacaaa tggcaaccat ggtacgatga tgatccagac atgataagat
     7681 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7741 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7801 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     7861 gcaagtaaaa cctctacaaa tgtggtatgg ctgattatga tcctctagat cagatctctt
     7921 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7981 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     8041 tgtctggatc tacgtaatac gactcactat agttctagag gctcgagagg ggccgcttta
     8101 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
     8161 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
     8221 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     8281 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     8341 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     8401 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     8461 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     8521 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     8581 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     8641 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     8701 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     8761 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     8821 cactgtctgc tttgctgttg gtctgggctc ctgggtcact ggcttactaa tggagtcttt
     8881 atgtatgagg actcttatca attgttcttc tataaaggtc tgcagtgttt ctgttcgtcc
     8941 cctacatgga cacccagagc ctcctaaata caggagccct gataactgca caagtgctca
     9001 gattccagca gggtggaaaa tgagataaag tgtgcagatg gggaggggga cgtgaatgag
     9061 agatttgagg gatgaaaagg atggatgaac gcattgaaaa tagccccttt cacaaaataa
     9121 taccagtaaa ttgtcatata attaatgacc agacctttac ctgtaaatga tgtgatgttc
     9181 atatacagaa gaatgccaac aatttacaga tgattttaca aattcttaat tttccttttc
     9241 taaatggatt tgttcacaca ttatctcttc aaagcaattg aattttctgg aaaagattgg
     9301 ctgtgtgtgt aaaaggtgct actgaggaat ttaatgtgac atggaatgaa gcaaacagca
     9361 gcctaagaca ggatggcagg aaaaatgtca catgtttaaa ataagagtga aaccaacgac
     9421 ctggacttat agagactgtg tgtccttagg caaagctctt gtacaaacag tgcttgtgac
     9481 tgtgaaattc ggggtttgcc tggattgtgt taaatgtgtg cttggatgta tcataaaaaa
     9541 gaatcggttc tctgtatttg acattttctg taatttatct aaagttacaa ctggctatgc
     9601 ctgattttta tcactattga atcaatcatt ttaaagaatg ttaagtctgc tgatttggag
     9661 agctcattta catttatttt gaatgtctct tatttaacac aactgattta agctttagat
     9721 ctaatctgat cattcaggcg cgccaacacg tgaataccac gcgaggcctt agggataaca
     9781 gggtaatacg cgtgatctgc gaagatacgg ccacgggtgc tcttgatcct gtggctgatt
     9841 ttggactgtg ctgctcgcag ctgctgatga atcacatact tcctccattt tcttccactg
     9901 attgactgtt ataatttccc taatttccag gtcaaggtgc tgtgcattgt ggtaatagat
     9961 gtgacatgac gtcacttcca aaggaccaat gaacatgtct gaccaatttc atataatgtg
    10021 aaaacgattt tcataggcag aataaataac atttaaatta aactgggcat cagcgcaatt
    10081 caattggttt ggtaatagca agggaaaata gaatgaagtg atctccaaaa aataagtact
    10141 ttttgactgt aaataaaatt gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa
    10201 ttaagtaaaa gtaaaagtat tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa
    10261 aataatactt aagtacagta atcaagtaaa attactcaag tactttacac ctctggttct
    10321 tgacccccta ccttcagcaa gcccagcaga tccactagtt ctagagcggc cgccaccgcg
    10381 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
    10441 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
    10501 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
    10561 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10621 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10681 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10741 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10801 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10861 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10921 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10981 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    11041 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    11101 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    11161 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    11221 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    11281 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    11341 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    11401 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    11461 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    11521 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    11581 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11641 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11701 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11761 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11821 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11881 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11941 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    12001 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    12061 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    12121 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    12181 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    12241 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    12301 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    12361 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    12421 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    12481 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    12541 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtg
//
