LOCUS       sequence-147497-       10885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Circuit plasmid: 0x1C.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10885)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 10885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pAN3944
FEATURES             Location/Qualifiers
     source          1..10885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(308..852)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(388..407)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(941..1756)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(941..1756)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1664..1683
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1748..1767
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     promoter        3765..4049
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    4893..4911
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4918..4936
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        5772..6056
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     protein_bind    6097..6115
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6122..6140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        join(6949..6954,6955..6970,6971..6977)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    6983..7002
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        7863..7897
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     RBS             7906..7917
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             8773..8784
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             8902..9984
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8921..8940)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             10003..10626
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(10076..10095)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(10809..10828)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcttcctcgc tcactgactc gctgcacgag gcagacctca gcgctagcgg agtgtatact
       61 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
      121 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
      181 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
      241 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
      301 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
      361 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttcccctg gcggctccct
      421 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      481 tttgtctcat tccacgcctg acactcagtt ccgggtaggc agttcgctcc aagctggact
      541 gtatgcacga accccccgtt cagtccgacc gctgcgcctt atccggtaac tatcgtcttg
      601 agtccaaccc ggaaagacat gcaaaagcac cactggcagc agccactggt aattgattta
      661 gaggagttag tcttgaagtc atgcgccggt taaggctaaa ctgaaaggac aagttttggt
      721 gactgcgctc ctccaagcca gttacctcgg ttcaaagagt tggtagctca gagaaccttc
      781 gaaaaaccgc cctgcaaggc ggttttttcg ttttcagagc aagagattac gcgcagacca
      841 aaacgatctc aagaagatca tcttattaag gggtctgacg ctcagtggaa cgaaaaatca
      901 atctaaagta tatatgagta aacttggtct gacagttacc ttagaaaaac tcatcgagca
      961 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
     1021 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
     1081 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
     1141 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
     1201 aaagcttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
     1261 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
     1321 cgcgatcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
     1381 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
     1441 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
     1501 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
     1561 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
     1621 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
     1681 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctcgagcaa gacgtttccc
     1741 gttgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac agttttattg
     1801 ttcatgatga tatattttta tcttgtgcaa tgtacatcag agattttgag acacaaccaa
     1861 ttattgaagg cctccctaac ggggggcctt tttttgtttc tggtctcccg ctttctatga
     1921 ttggtccaga ttcgttacca attgacagct agctcagtcc taggtatata catacatgct
     1981 tgtttgtttg taaaccttgt ccaaccaaat gattcgttac caattgacag tttctatcga
     2041 tctatagata atgctagcct gaagcggtca acgcatgtgc tttgcgttct gatgagacag
     2101 tgatgtcgaa accgcctcta caaataattt tgtttaactt tacgagggcg atcctatggc
     2161 acgtaccccg agccgtagca gcattggtag cctgcgtagt ccgcataccc ataaagcaat
     2221 tctgaccagc accattgaaa tcctgaaaga atgtggttat agcggtctga gcattgaaag
     2281 cgttgcacgt cgtgccggtg caagcaaacc gaccatttat cgttggtgga ccaataaagc
     2341 agcactgatt gccgaagtgt atgaaaatga aagcgaacag gtgcgtaaat ttccggatct
     2401 gggtagcttt aaagccgatc tggattttct gctgcgtaat ctgtggaaag tttggcgtga
     2461 aaccatttgt ggtgaagcat ttcgttgtgt tattgcagaa gcacagctgg accctgcaac
     2521 cctgacccag ctgaaagatc agtttatgga acgtcgtcgt gagatgccga aaaaactggt
     2581 tgaaaatgcc attagcaatg gtgaactgcc gaaagatacc aatcgtgaac tgctgctgga
     2641 tatgattttt ggtttttgtt ggtatcgcct gctgaccgaa cagctgaccg ttgaacagga
     2701 tattgaagaa tttaccttcc tgctgattaa tggtgtttgt ccgggtacac agcgttaagg
     2761 aaacacagaa aaaagcccgc acctgacagt gcgggctttt tttttcgacc aaaggtgtca
     2821 atccgcgtga taggtctgat tcgttaccaa ttgacggaat gaacgttcat tccgataatg
     2881 ctagcctgaa gcgctcaacg ggtgtgcttc ccgttctgat gagtccgtga ggacgaaagc
     2941 gcctctacaa ataattttgt ttaagagtct atggactatg ttttcacata tgagatacca
     3001 ggatggcacg taaaaccgca gcagaagcag aagaaacccg tcagcgtatt attgatgcag
     3061 cactggaagt ttttgttgca cagggtgtta gtgatgcaac cctggatcag attgcacgta
     3121 aagccggtgt tacccgtggt gcagtttatt ggcattttaa tggtaaactg gaagttctgc
     3181 aggcagttct ggcaagccgt cagcatccgc tggaactgga ttttacaccg gatctgggta
     3241 ttgaacgtag ctgggaagca gttgttgttg caatgctgga tgcagttcat agtccgcaga
     3301 gcaaacagtt tagcgaaatt ctgatttatc agggtctgga tgaaagcggt ctgattcata
     3361 atcgtatggt tcaggcaagc gatcgttttc tgcagtatat tcatcaggtt ctgcgtcatg
     3421 cagttaccca gggtgaactg ccgattaatc tggatctgca gaccagcatt ggtgttttta
     3481 aaggtctgat taccggtctg ctgtatgaag gtctgcgtag caaagatcag caggcacaga
     3541 ttatcaaagt tgcactgggt agcttttggg cactgctgcg tgaaccgcct cgttttctgc
     3601 tgtgtgaaga agcacagatt aaacaggtga aatccttcga ataattcagc caaaaaactt
     3661 aagaccgccg gtcttgtcca ctaccttgca gtaatgcggt ggacaggatc ggcggttttc
     3721 ttttctcttc tcaatctgac ttttcatact cccgccattc agagaagaaa ccaattgtcc
     3781 atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc taaccaaacc
     3841 ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca tgacaaaaac
     3901 gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt tgcacggcgt
     3961 cacactttgc tatgccatag catttttatc cataagatta gcggatccta cctgacgctt
     4021 tttatcgcaa ctctctactg tttctccata cccgtttttt tgggctagca ccaggaatct
     4081 gaacgattcg ttaccaattg acatatttaa aattcttgtt taaaatgcta gcctgagact
     4141 gtcgccggat gtgtatccga cctgacgatg gcccaaaagg gccgaaacag tcctctacaa
     4201 ataattttgt ttaaccaaac gaggccggga ggatggaaag caccccgacc aaacagaaag
     4261 caatttttag cgcaagcctg ctgctgtttg cagaacgtgg ttttgatgca accaccatgc
     4321 cgatgattgc agaaaatgca aaagttggtg caggcaccat ttatcgctat ttcaaaaaca
     4381 aagaaagcct ggtgaacgaa ctgtttcagc agcatgttaa tgaatttctg cagtgtattg
     4441 aaagcggtct ggcaaatgaa cgtgatggtt atcgtgatgg ctttcatcac atttttgaag
     4501 gtatggtgac ctttaccaaa aatcatccgc gtgcactggg ttttatcaaa acccatagcc
     4561 agggcacctt tctgaccgaa gaaagccgtc tggcatatca gaaactggtt gaatttgtgt
     4621 gcaccttttt tcgtgaaggt cagaaacagg gtgtgattcg taatctgccg gaaaatgcac
     4681 tgattgcaat tctgtttggc agctttatgg aagtgtatga aatgatcgag aacgattatc
     4741 tgagcctgac cgatgaactg ctgaccggtg ttgaagaaag cctgtgggca gcactgagcc
     4801 gtcagagcta actcggtacc aaattccaga aaagagacgc tttcgagcgt cttttttcgt
     4861 tttggtccgg gctactccac cgttggcttt tttccctatc agtgatagag attgacatcc
     4921 ctatcagtga tagagataat gagcacacca ggaatctgaa cgattcgtta ccaattgaca
     4981 tatttaaaat tcttgtttaa aatgctagcc tgaagaagtc aattaatgtg cttttaattc
     5041 tgatgagtcg gtgacgacga aacttcctct acaaataatt ttgtttaacc ccccgaggag
     5101 tagcacatgc cgaaactggg tatgcagagc attcgtcgtc gtcagctgat tgatgcaacc
     5161 ctggaagcaa ttaatgaagt tggtatgcat gatgcaacca ttgcacagat tgcacgtcgt
     5221 gccggtgtta gcaccggtat tattagccat tatttccgcg ataaaaacgg tctgctggaa
     5281 gcaaccatgc gtgatattac cagccagctg cgtgatgcag ttctgaatcg tctgcatgca
     5341 ctgccgcagg gtagcgcaga acagcgtctg caggcaattg ttggtggtaa ttttgatgaa
     5401 acccaggtta gcagcgcagc aatgaaagca tggctggcat tttgggcaag cagcatgcat
     5461 cagccgatgc tgtatcgtct gcagcaggtt agcagtcgtc gtctgctgag caatctggtt
     5521 agcgaatttc gtcgtgaact gcctcgtgaa caggcacaag aggcaggtta tggtctggca
     5581 gcactgattg atggtctgtg gctgcgtgca gcactgagcg gtaaaccgct ggataaaacc
     5641 cgtgcaaata gcctgacccg tcattttatc acccagcatc tgccgaccga ttaaccaatt
     5701 attgaacacc cttcggggtg tttttttgtt tctggtctac ccgctacttt tcatactccc
     5761 gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt
     5821 ttactggctc ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca
     5881 aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa
     5941 agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat
     6001 aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc
     6061 gtttttttgg gctagctact ccaccgttgg cttttttccc tatcagtgat agagattgac
     6121 atccctatca gtgatagaga taatgagcac ctgaagtagt caccggctgt gcttgccggt
     6181 ctgatgagcc tgtgaaggcg aaactacctc tacaaataat tttgtttaaa cccccgagat
     6241 gaaatacatc ctgtttgagg tgtgcgaaat gggtaaaagc cgtgaacaga ccatggaaaa
     6301 tattctgaaa gcagccaaaa agaaattcgg cgaacgtggt tatgaaggca ccagcattca
     6361 agaaattacc aaagaagcca aagttaacgt tgcaatggcc agctattact ttaatggcaa
     6421 agagaacctg tactacgagg tgttcaaaaa atacggtctg gcaaatgaac tgccgaactt
     6481 tctggaaaaa aaccagttta atccgattaa tgccctgcgt gaatatctga ccgtttttac
     6541 cacccacatt aaagaaaatc cggaaattgg caccctggcc tatgaagaaa ttatcaaaga
     6601 aagcgcacgc ctggaaaaaa tcaaaccgta ttttatcggc agcttcgaac agctgaaaga
     6661 aattctgcaa gagggtgaaa aacagggtgt gtttcacttt tttagcatca accataccat
     6721 ccattggatt accagcattg ttctgtttcc gaaattcaaa aaattcatcg atagcctggg
     6781 tccgaatgaa accaatgata ccaatcatga atggatgccg gaagatctgg ttagccgtat
     6841 tattagcgca ctgaccgata aaccgaacat ttaaaacgca tgagaaagcc cccggaagat
     6901 caccttccgg gggctttttt attgcgcagg taacgatcgt tggctgtgtt gacaattaat
     6961 catcggctcg tataatgtgt ggaattgtga gcgctcacaa ttctgaaggg tgtctcaagg
     7021 tgcgtacctt gactgatgag tccgaaagga cgaaacaccc ctctacaaat aattttgttt
     7081 aaaatgttcc ctaataatca gcaaagaggt tactagatgg caggcgcagt tggtcgtccg
     7141 cgtcgtagtg caccgcgtcg tgcaggtaaa aatccgcgtg aagaaattct ggatgcaagc
     7201 gcagaactgt ttacccgtca gggttttgca accaccagta cccatcagat tgcagatgca
     7261 gttggtattc gtcaggcaag cctgtattat cattttccga gcaaaaccga aatctttctg
     7321 accctgctga aaagcaccgt tgaaccgagc accgttctgg cagaagatct gagcaccctg
     7381 gatgcaggtc cggaaatgcg tctgtgggca attgttgcaa gcgaagttcg tctgctgctg
     7441 agcaccaaat ggaatgttgg tcgtctgtat cagctgccga ttgttggtag cgaagaattt
     7501 gcagaatatc atagccagcg tgaagcactg accaatgttt ttcgtgatct ggcaaccgaa
     7561 attgttggtg atgatccgcg tgcagaactg ccgtttcata ttaccatgag cgttattgaa
     7621 atgcgtcgca atgatggtaa aattccgagt ccgctgagcg cagatagcct gccggaaacc
     7681 gcaattatgc tggcagatgc aagcctggca gttctgggtg caccgctgcc tgcagatcgt
     7741 gttgaaaaaa ccctggaact gattaaacag gcagatgcaa aataactcgg taccaaagac
     7801 gaacaataag acgctgaaaa gcgtcttttt tcgttttggt ccaatggtca ccatatatca
     7861 agtttacggc tagctcagtc ctaggtacta tgctagctac tagagaaaga ggagaaatac
     7921 tagatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg
     7981 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg
     8041 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat
     8101 cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc aggagagatt
     8161 catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt
     8221 ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa tacggggttc
     8281 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac
     8341 gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg
     8401 ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta
     8461 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc
     8521 gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta
     8581 gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa gctgcttttg
     8641 agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc
     8701 tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt
     8761 taataaccaa ttattgaagg ccgctaacgc agcctttttt tgtttctggt ctcccaatgg
     8821 cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag
     8881 agtcaattca gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc
     8941 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
     9001 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
     9061 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
     9121 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
     9181 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
     9241 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
     9301 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
     9361 acacccatca acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat
     9421 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
     9481 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
     9541 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
     9601 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
     9661 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
     9721 gacgataccg aagatagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
     9781 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     9841 aagggcaatc agctgttgcc agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     9901 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     9961 tcccgactgg aaagcgggca gtgacaatcc aggaggaaaa aaatgtccag attagataaa
    10021 agtaaagtga ttaacagcgc attagagctg cttaatgagg tcggaatcga aggtttaaca
    10081 acccgtaaac tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa
    10141 aataagcggg ctttgctcga cgccttagcc attgagatgt tagataggca ccatactcac
    10201 ttttgccctt tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt
    10261 agatgtgctt tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca
    10321 gaaaaacagt atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca
    10381 ctagagaatg cattatatgc actcagcgct gtggggcatt ttactttagg ttgcgtattg
    10441 gaagatcaag agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg
    10501 ccgccattat tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc
    10561 ttattcggcc ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg
    10621 tcctaataat tggtaacgaa tcagacaatt gacggctcga gggagtagca tagggtttgc
    10681 agaatccctg cttcgtccat ttgacaggca cattatgcat cgatgataag ctgtcaaaca
    10741 tgagcagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
    10801 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
    10861 catgagcaaa tattttatct gaggt
//
