LOCUS       sequence-147510-         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Post cloned 4-piece from Isothermal/Golden Gate assembly (GGA), 
            using sequences from 2 (PltetO1-UTR1), 3 (tetR+linker from primer 
            extension), 21 (linker from primer extension + deGFP), and 105 
            (vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Sun ZZ, Yeung E, Hayes CA, Noireaux V, Murray RM
  TITLE     Linear DNA for rapid prototyping of synthetic biological circuits in
            an Escherichia coli based TX-TL cell-free system.
  JOURNAL   ACS Synth Biol. 2014 Jun 20;3(6):387-97. doi: 10.1021/sb400131a. 
            Epub 2013 Dec 4.
  PUBMED    24303785
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb400131a";
            journalName: "ACS Synth Biol"; date: "2014-06-20- 20"; volume: "3"; 
            issue: "6"; pages: "387-97"
COMMENT     Sequence Label: pBEST-Pl-tetO1-tetR-linker-deGFP-T500 (109p)
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(201..222)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     550..569
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     809..830
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(870..890)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
       61 agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt
      121 gaaggctctc aagggcatcg gtcgacacag aaaagcccgc ctttcggcgg gctttgctcg
      181 agttagatcc cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg
      241 gggtctttgc tcagggcgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg
      301 ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg
      361 atgttgtggc ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata
      421 tagacgttgt ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc
      481 ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc
      541 tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag
      601 ccttcgggca tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg
      661 aagcactgca cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg
      721 ccggtggtgc agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg
      781 ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggaaca
      841 acgccagtga aaagctccat agcaccacca gactggaagt acaggttttc accagaccca
      901 ct
//
