LOCUS       sequence-147522-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive ETV4 ORF, blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pLX_TRC311 ETV4-S
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(74..115)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    123..147
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(769..793)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(845..865)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgact taacgcgttg attgtcgact taaccggtac
       61 tagttcatta ctacgtagaa tcgagaccga ggagagggtt agggataggc ttaccgatat
      121 caaccacttt gtacaagaaa gttgggtagt aagagtagcc acccttgggg ccaaatggct
      181 gggcggggcc agccagctct gggaggtagg cggggctctc atccaagtgg gacaaaggga
      241 ctgtgtcctc ctcactgaca ggccggtcaa actcagcctt gagagctgga cgctgattgt
      301 ccgggaaggc caaagagaag agggcctcgg gctcacacac aaacttgtac acgtaacgct
      361 caccagccac cttctgcatg atgcctttct cataatagta tcggagcgag cggctcagct
      421 tgtcgtaatt catggctggc cggttcttct ggatgcccca gagcctggcg acctcctcag
      481 gctcaatgag cttgaactcc attccccggc ccgtccaggc aatgaaatgg gcatttgttg
      541 ggtcatccag caaggccacc agaaattgcc acagctgcag ggcaccccgg cgctggtagg
      601 gcggcccctc tcgaaatgca ccgacccctt cctgcttgat gtctccttca aatttctcag
      661 ggacaacgca gacatcatct gggaatggtc gcagaggttt ctcatagcca tagcccatgg
      721 ccccgtcacc tggagagggc ccagagaagc cctctgtgtg gaggtacatg ccaacttttt
      781 tgtacaaact tgttgatcaa tcgatgctag cctcacgaca cctgaaatgg aagaaaaaaa
      841 ctttgaacca ctgtctgagg cttgagaatg aaccaagatc caaactcaaa aagggcaaa
//
