LOCUS       sequence-147527-         749 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  retroviral shRNA vector against human HOIP/RNF31, expresses GFP for 
            transduction control.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Gabriel CH, Gross F, Karl M, Stephanowitz H, Hennig AF, Weber M, 
            Gryzik S, Bachmann I, Hecklau K, Wienands J, Schuchhardt J, Herzel 
            H, Radbruch A, Krause E, Baumgrass R
  TITLE     Identification of Novel Nuclear Factor of Activated T Cells 
            (NFAT)-Associated Proteins in T cells.
  JOURNAL   J Biol Chem. 2016 Sep 16. pii: jbc.M116.739326.
  PUBMED    27637333
REFERENCE   2  (bases 1 to 749)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2016 Sep 16. pii: jbc.M116.739326."
COMMENT     Sequence Label: pQCXIX_sh_HOIP/RNF31_#6
FEATURES             Location/Qualifiers
     source          1..749
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(52..364)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(113..133)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 aaagctgtgc tatgttccat attctcttga attatggaac atagcacagc aaacaaggct
       61 tttctccaag ggatatttat agtctcaaaa cacacaatta ctttacagtt agggtgagtt
      121 tccttttgtg ctgtttttta aaataataat ttagtatttg tatctcttat agaaatccaa
      181 gcctatcatg taaaatgtag ctagtattaa aaagaacaga ttatctgtct tttatcgcac
      241 attaagcctc tatagttact aggaaatatt atatgcaaat taaccggggc aggggagtag
      301 ccgagcttct cccacaagtc tgtgcgaggg ggccggcgcg ggcctagaga tggcgcgtcg
      361 gatctctaga gcgggcccgg tactaaccaa actggatctc tgctcgaccc tgttccatct
      421 gttcctgacc ttgatctgaa cttctctatt ctcagttatg tatttttcca tgccttgcaa
      481 aatggcgtta cttaagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
      541 ttctggagac taaataaaat cttttatttt atctatggct cgtactctat aggcttcagc
      601 tggtgatatt gttgagtcaa aactagagcc tggaccactg atatccatat gactagtccc
      661 cgaagcttga gctcgactag ctagctagct actagctagt cgagatctga gtccggactt
      721 gtacagctcg tccatgccga gagtgatcc
//
