LOCUS       sequence-147529-       11521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11521)
  AUTHORS   Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA
  TITLE     Control of differentiation in a self-renewing mammalian tissue by 
            the histone demethylase JMJD3.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1865-70.
  PUBMED    18628393
REFERENCE   2  (bases 1 to 11521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1865-70."
COMMENT     Sequence Label: MSCV JMJD3
FEATURES             Location/Qualifiers
     source          1..11521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2026..2044
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2082..2104)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2204..2223
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2417..2439
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2567..2586
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             2786..3302
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    3366..3707
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             3774..4190
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4120..4142
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4158..4174
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      4203..4212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4212..4235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4248..4274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    4290..4314
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4735..4746)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5095..5121
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             6585..6614
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             6588..6617
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(9408..9432)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    9445..10012
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    9462..10014
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9606..9623)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9833..9852
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10026..10625
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10026..10045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10522..10542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             10701..11215
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    11377..11393
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11401..11407,11408..11425,11426..11431))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11446..11467
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
     2041 aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
     2101 ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
     2161 gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt
     2221 gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac
     2281 cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     2341 gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg
     2401 gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat
     2461 gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga
     2521 aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga
     2581 tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt
     2641 agaggcgatt agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga
     2701 ctcaacaata tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt
     2761 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
     2821 cttaagtaac gccattttgc aaggcatgga aaatacataa ctgagaatag agaagttcag
     2881 atcaaggtta ggaacagaga gacagcagaa tatgggccaa acaggatatc tgtggtaagc
     2941 agttcctgcc ccggctcagg gccaagaaca gatggtcccc agatgcggtc ccgccctcag
     3001 cagtttctag agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt
     3061 gccttatttg aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc
     3121 cgagctcaat aaaagagccc acaacccctc actcggcgcg ccagtcctcc gatagactgc
     3181 gtcgcccggg tacccgtatt cccaataaag cctcttgctg tttgcatccg aatcgtggac
     3241 tcgctgatcc ttgggagggt ctcctcagat tgattgactg cccacctcgg gggtctttca
     3301 tttggaggtt ccaccgagat ttggagaccc ctgcccaggg accaccgacc cccccgccgg
     3361 gaggtaagct ggccagcggt cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg
     3421 gcatctaatg tttgcgcctg cgtctgtact agttagctaa ctagctctgt atctggcgga
     3481 cccgtggtgg aactgacgag ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg
     3541 actttggggg ccgtttttgt ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc
     3601 cgtcaggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     3661 aatttttgct ttcggtttgg aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca
     3721 tcgttctgtg ttgtctctgt ctgactgtgt ttctgtattt gtctgaaaat tagggccaga
     3781 ctgttaccac tcccttaagt ttgaccttag gtcactggaa agatgtcgag cggatcgctc
     3841 acaaccagtc ggtagatgtc aagaagagac gttgggttac cttctgctct gcagaatggc
     3901 caacctttaa cgtcggatgg ccgcgagacg gcacctttaa ccgagacctc atcacccagg
     3961 ttaagatcaa ggtcttttca cctggcccgc atggacaccc agaccaggtc ccctacatcg
     4021 tgacctggga agccttggct tttgaccccc ctccctgggt caagcccttt gtacacccta
     4081 agcctccgcc tcctcttcct ccatccgccc cgtctctccc ccttgaacct cctcgttcga
     4141 ccccgcctcg atcctccctt tatccagccc tcactccttc tctaggcgcc ggaattagat
     4201 ctgccaccat ggactacaag gatgacgatg acaagctcga tggaggatac ccatacgatg
     4261 ttccagatta cgctgctcga ggttatcaaa caagtttgta caaaaaagca ggctccgcgg
     4321 ccgccccctt caccggatcc atgcatcggg cagtggatcc tccaggggcc cgcgctgcac
     4381 gggaagcctt tgcccttggg ggcctgagct gtgctggggc ctggagctcc tgcccgcctc
     4441 atccccctcc tcgtagcgca tggctgcctg gaggcagatg ctcagccagc attgggcagc
     4501 ccccgcttcc tgctccccta cccccttcac atggcagtag ttctgggcac cccagcaaac
     4561 catattatgc tccaggggcg cccactccaa gacccctcca tgggaagctg gaatccctgc
     4621 atggctgtgt gcaggcattg ctccgggagc cagcccagcc agggctttgg gaacagcttg
     4681 ggcaactgta cgagtcagag cacgatagtg aggaggccac acgctgctac cacagcgccc
     4741 ttcgatacgg aggaagcttc gctgagctgg ggccccgcat tggccgactg cagcaggccc
     4801 agctctggaa ctttcatact ggctcctgcc agcaccgagc caaggtcctg cccccactgg
     4861 agcaagtgtg gaacttgcta caccttgagc acaaacggaa ctatggagcc aagcggggag
     4921 gtcccccggt gaagcgagct gctgaacccc cagtggtgca gcctgtgcct cctgcagcac
     4981 tctcaggccc ctcaggggag gagggcctca gccctggagg caagcgaagg agaggctgca
     5041 actctgaaca gactggcctt cccccagggc tgccactgcc tccaccacca ttaccaccac
     5101 caccaccacc accaccacca cccctgcctg gcctggctac cagcccccca tttcagctaa
     5161 ccaagccagg gctgtggagt accctgcatg gagatgcctg gggcccagag cgcaagggtt
     5221 cagcaccccc agagcgccag gagcagcggc actcgctgcc tcacccatat ccatacccag
     5281 ctccagcgta caccgcgcac ccccctggcc accggctggt cccggctgct cccccaggcc
     5341 caggcccccg ccccccagga gcagagagcc atggctgcct gcctgccacc cgtccccccg
     5401 gaagtgacct tagagagagc agagttcaga ggtcgcggat ggactccagc gtttcaccag
     5461 cagcaaccac cgcctgcgtg ccttacgccc cttcccggcc ccctggcctc cccggcacca
     5521 ccaccagcag cagcagtagc agcagcagca acactggtct ccggggcgtg gagccgaacc
     5581 caggcattcc cggcgctgac cattaccaaa ctcccgcgct ggaggtctct caccatggcc
     5641 gcctggggcc ctcggcacac agcagtcgga aaccgttctt gggggctccc gctgccactc
     5701 cccacctatc cctgccacct ggaccttcct cacccccccc cccccccctg tccccgcctc
     5761 ttacgccccc caccaccccc tgcctggttg aagggtccgg cctgccgggc agcccgagag
     5821 gatggagaga tcttagaaga gctcttcttt gggactgagg gacccccccg ccctgcccca
     5881 ccacccctcc cccatcgcga gggcttcttg gggcctccgg cctcccgctt ttctgtgggc
     5941 actcaggatt ctcacacccc tcccactccc ccaaccccaa ccaccagcag tagcaacagc
     6001 aacagtggca gccacagcag cagccctgct gggcctgtgt cctttccccc accaccctat
     6061 ctggccagaa gtatagaccc ccttccccgg cctcccagcc cagcacagaa cccccaggac
     6121 ccacctcttg tacccctgac tcttgccctg cctccagccc ctccttcctc ctgccaccaa
     6181 aatacctcag gaagcttcag gcgcccggag agcccccggc ccagggtctc cttcccaaag
     6241 acccccgagg tggggccggg gccaccccca ggccccctga gtaaagcccc ccagcctgtg
     6301 ccgcccgggg ttggggagct gcctgcccga ggccctcgac tctttgattt tccccccact
     6361 ccgctggagg accagtttga ggagccagcc gaattcaaga tcctacctga tgggctggcc
     6421 aacatcatga agatgctgga cgaatccatt cgcaaggaag aggaacagca acaacacgaa
     6481 gcaggcgtgg ccccccaacc cccgctgaag gagccctttg catctctgca gtctcctttc
     6541 cccaccgaca cagcccccac cactactgct cctgctgtcg ccgtcaccac caccaccacc
     6601 accaccacca ccaccacggc cacccaggaa gaggagaaga agccaccacc agccctacca
     6661 ccaccaccgc ctctagccaa gttccctcca ccctctcagc cacagccacc accaccccca
     6721 ccccccagcc cggccagcct gctcaaatcc ttggcctccg tgctggaggg acaaaagtac
     6781 tgttatcggg ggactggagc agctgtttcc acccggcctg ggcccttgcc caccactcag
     6841 tattcccctg gccccccatc aggtgctacc gccctgccgc ccacctcagc ggcccctagc
     6901 gcccagggct ccccacagcc ctctgcttcc tcgtcatctc agttctctac ctcaggcggg
     6961 ccctgggccc gggagcgcag ggcgggcgaa gagccagtcc cgggccccat gacccccacc
     7021 caaccgcccc cacccctatc tctgccccct gctcgctctg agtctgaggt gctagaagag
     7081 atcagccggg cttgcgagac ccttgtggag cgggtgggcc ggagtgccac tgacccagcc
     7141 gacccagtgg acacagcaga gccagcggac agtgggactg agcgactgct gccccccgca
     7201 caggccaagg aggaggctgg cggggtggcg gcagtgtcag gcagctgtaa gcggcgacag
     7261 aaggagcatc agaaggagca tcggcggcac aggcgggcct gtaaggacag tgtgggtcgt
     7321 cggccccgtg agggcagggc aaaggccaag gccaaggtcc ccaaagaaaa gagccgccgg
     7381 gtgctgggga acctggacct gcagagcgag gagatccagg gtcgtgagaa gtcccggccc
     7441 gatcttggcg gggcctccaa ggccaagcca cccacagctc cagcccctcc atcagctcct
     7501 gcaccttctg cccagcccac acccccgtca gcctctgtcc ctggaaagaa ggctcgggag
     7561 gaagccccag ggccaccggg tgtcagccgg gccgacatgc tgaagctgcg ctcacttagt
     7621 gaggggcccc ccaaggagct gaagatccgg ctcatcaagg tagagagtgg tgacaaggag
     7681 acctttatcg cctctgaggt ggaagagcgg cggctgcgca tggcagacct caccatcagc
     7741 cactgtgctg ctgacgtcgt gcgcgccagc aggaatgcca aggtgaaagg gaagtttcga
     7801 gagtcctacc tttcccctgc ccagtctgtg aaaccgaaga tcaacactga ggagaagctg
     7861 ccccgggaaa aactcaaccc ccctacaccc agcatctatc tggagagcaa acgggatgcc
     7921 ttctcacctg tcctgctgca gttctgtaca gaccctcgaa atcccatcac agtgatccgg
     7981 ggcctggcgg gctccctgcg gctcaacttg ggcctcttct ccaccaagac cctggtggaa
     8041 gcgagtggcg aacacaccgt ggaagttcgc acccaggtgc agcagccctc agatgagaac
     8101 tgggatctga caggcactcg gcagatctgg ccttgtgaga gctcccgttc ccacaccacc
     8161 attgccaagt acgcacagta ccaggcctca tccttccagg agtctctgca ggaggagaag
     8221 gagagtgagg atgaggagtc agaggagcca gacagcacca ctggaacccc tcctagcagc
     8281 gcaccagacc cgaagaacca tcacatcatc aagtttggca ccaacatcga cttgtctgat
     8341 gctaagcggt ggaagcccca gctgcaggag ctgctaaagc tgcccgcctt catgcgggta
     8401 acatccacgg gcaacatgct gagccacgtg ggccacacca tcctgggcat gaacacggtg
     8461 cagctgtaca tgaaggtgcc cggcagccga acgccaggcc accaggagaa taacaacttc
     8521 tgctccgtca acatcaacat tggcccaggc gactgcgagt ggttcgcggt gcacgagcac
     8581 tactgggaga ccatcagcgc tttctgtgat cggcacggcg tggactactt gacgggttcc
     8641 tggtggccaa tcctggatga tctctatgca tccaatattc ctgtgtaccg cttcgtgcag
     8701 cgacccggag acctcgtgtg gattaatgcg gggactgtgc actgggtgca ggccaccggc
     8761 tggtgcaaca acattgcctg gaacgtgggg cccctcaccg cctatcagta ccagctggcc
     8821 ctggaacgat acgagtggaa tgaggtgaag aacgtcaaat ccatcgtgcc catgattcac
     8881 gtgtcatgga acgtggctcg cacggtcaaa atcagcgacc ccgacttgtt caagatgatc
     8941 aagttctgcc tgctgcagtc catgaagcac tgccaggtgc aacgcgagag cctggtgcgg
     9001 gcagggaaga aaatcgctta ccagggccgt gtcaaggacg agccagccta ctactgcaac
     9061 gagtgcgatg tggaggtgtt taacatcctg ttcgtgacaa gtgagaatgg cagccgcaac
     9121 acgtacctgg tacactgcga gggctgtgcc cggcgccgca gcgcaggcct gcagggcgtg
     9181 gtggtgctgg agcagtaccg cactgaggag ctggctcagg cctacgacgc cttcacgctg
     9241 gtgagggccc ggcgggcgcg cgggcagcgg aggagggcac tggggcaggc tgcagggacg
     9301 ggcttcggga gcccggccgc gcctttccct gagcccccgc cggctttctc cccccaggcc
     9361 ccagccagca cgtcgcgatg actcgaggcg aaagggtggg cgcgccgacc cagctttctt
     9421 gtacaaagtg gttcgataac gaattccgcc ccccccccct aacgttactg gccgaagccg
     9481 cttggaataa ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt
     9541 tggcaatgtg agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct
     9601 ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct
     9661 ggaagcttct tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc
     9721 acctggcgac aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc
     9781 ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc
     9841 ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc
     9901 tgatctgggg cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct
     9961 aggccccccg aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca
    10021 caaccatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg
    10081 ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc
    10141 cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc
    10201 tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc
    10261 cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga
    10321 gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca
    10381 aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc
    10441 tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct
    10501 tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca
    10561 ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg
    10621 cctgatgcat cgatgtcgac ctgcagccaa gcttatcgat aaaataaaag attttattta
    10681 gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag
    10741 taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt tcagatcaag
    10801 gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt aagcagttcc
    10861 tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc tcagcagttt
    10921 ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc ctgtgcctta
    10981 tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc tccccgagct
    11041 caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga ctgcgtcgcc
    11101 cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg gtctcgctgt
    11161 tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct ttcatgggta
    11221 acagtttctt gaagttggag aacaacattc tgagggtagg agtcgaatat taagtaatcc
    11281 tgactcaatt agccactgtt ttgaatccac atactccaat actcctgaaa tagttcatta
    11341 tggacagcgc agaaagagct ggggagaatt gtgaaattgt tatccgctca caattccaca
    11401 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11461 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    11521 g
//
