LOCUS       sequence-147538-       11968 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila 
            cell culture. Please note- this plasmid does not express GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11968)
  AUTHORS   Chavez A, Tuttle M, Pruitt BW, Ewen-Campen B, Chari R, Ter-Ovanesyan
            D, Haque SJ, Cecchi RJ, Kowal EJ, Buchthal J, Housden BE, Perrimon 
            N, Collins JJ, Church G
  TITLE     Comparison of Cas9 activators in multiple species.
  JOURNAL   Nat Methods. 2016 May 23. doi: 10.1038/nmeth.3871.
  PUBMED    27214048
REFERENCE   2  (bases 1 to 11968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 May 23. doi: 10.1038/nmeth.3871."
COMMENT     Sequence Label: pAct:dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..11968
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        140..2649
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     protein_bind    2667..2691
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      2697..2706
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2703..2729,2730..2732,2733..5216,5217..5222,5223..5288,
                     5289..5291,5292..6806)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=Cas9m4
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the mutations D10A, D839A, 
                     H840A, and N863A (Mali et al., 2013)"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVAAIVPQSFLKDDSIDNKVLTRSDKARGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             6819..6839
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6864..7013
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             7038..7058
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7488..7844
                     /codon_start=1
                     /product="transcriptional activation domain of human RelA, 
                     also known as p65 (O'Shea and Perkins, 2008)"
                     /label=RelA (p65) AD
                     /translation="PTQAGEGTLSEALLQLQFDDEDLGALLGNSTDPAVFTDLASVDNS
                     EFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGD
                     EDFSSIADMDFSALL"
     CDS             7863..8432
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
     protein_bind    complement(8437..8461)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      8469..8478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(8475..8477,8478..8480,8481..9191)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(8475..8477,8478..8480,8481..9191)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8520..8541)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(8781..8800)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9128..9149
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          9296..9361
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9491..9511
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9936..10070
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9973..9992)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10027..10046
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             join(10167..10235,10236..11027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10385..10404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11198..11786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11687..11706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11940..11957
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcaggcaact
       61 cgtgaaaggt aggcggatca gcggccgcag catgcaattc tatattctaa aaacacaaat
      121 gatacttcta aaaaaaaatc atgaatggca tcaactctga atcaaatctt tgcagatgca
      181 cctacttctc atttccactg tcacatcatt tttccagatc tcgctgcctg ttatgtggcc
      241 cacaaaccaa gacacgtttt atggccatta aagctggctg atcgtcgcca aacaccaaat
      301 acataatgaa tatgtacaca ttcgagaaag aagcgatcaa agaagcgtct tcgggcggag
      361 taggagaatg cggaggagaa ggagaacgag ctgatctagt atctctccac aatccaatgc
      421 caactgacca actggccata ttcggagcaa tttgaagcca atttccatcg cctggcgatc
      481 gctccattct tggctatatg tttttcaccg ttacccgggg ccattttcaa agactcgtcg
      541 gcaagataag attgtgtcac tcgctgtctc tcttcatttg tcgaagaatg ctgaggaatt
      601 tcgcgatgac gtcggcgagt attttgaaga atgagaataa tttgtattta tacgaaaatc
      661 agttagtgga attttctaca aaaacatgtt atctatagat aattttgttg caaaatatgt
      721 tgactatgac aaagattgta tgtatatacc tttaatgtat tctcattttc ttatgtattt
      781 ataatggcaa tgatgatact gatgatattt taagatgatg ccagaccaaa aggcttgaat
      841 ttctgcgtct tttgccgaac gcagtgcatg tgcaattgtt gttttttgga atattcaatt
      901 ttcggactgt ccgctttgat ttcagtttct tggcttattc aaaaagcaaa gtaaagccaa
      961 aaaagcgaga tggcaatacc aaatgcggca aaacggtagt ggaaggaaag gggtgcgggg
     1021 cagcggaagg aagggtgggg cggggcgtgg cggggtctgt ggctgggcgc gacgtcaccg
     1081 acgttggagc cactcctttg accatgtgtg cgtgtgtgta ttattcgtgt ctcgccactc
     1141 gccggttgtt tttttctttt tatgctgcgc tctctctagc gccatctcgc ttacgcatgc
     1201 tcaacgcacc gcatgttgcc gtttcctttt atgcgtcatt ttggctcgaa ataggcaatt
     1261 atttaaacaa agattagtca acgaaaacgc taaaataaat aagtctacaa tatggttact
     1321 tattgccatg tgtgtgcagc caacgatagc aacaaaagca acaacacagg tggctttccc
     1381 tctttcactt tttgtttgca agccgcgtgc gagcaagacg gcacgaccgg caaacgcaat
     1441 tacgctgaca aagagcagac gaagttttgg cgaaaaacat caaggcgcct gatacgaatg
     1501 catttgcaat aacaattgcg atatttaata ttgtttatga agctgtttga cttcaaaaca
     1561 cacaaaaaaa aaaataaaac aaattatttg aaagagaatt aggaatcgga cgcttatcgt
     1621 tagggtaaca acaagaaatg cttactgagt cacagcctct ggaaaactgc cgcaagccag
     1681 agagagagag aaaaagaggg agagcagctt agaccgcatg tgcttgtgtg tgaggcgtct
     1741 ctctcttcgt ctctgttgcg caaacgcata gactgcactg agaaaatcga ttacctattt
     1801 tttatgaatg aatatttgca ctattactat tcaaaactat taagatagca atcacattca
     1861 atagccaaat actataccac ctgagcgatg caacgaaatg atcaatttga gcaaaaatgc
     1921 tgcatattta ggacggcatc attatagaaa tgcttcttgc tgtgtacttt tctctcgtct
     1981 ggcagctgtt tcgccgttat tgttaaaacc ggcttaagtt aggtgtgttt tctacgacta
     2041 gtgaatgccc tactagaaga tgtgtgttgc acaaaatgtc cctggaataa ccaatttgaa
     2101 gtgcagatag cagtaaacgt aagctaatat gaatattatt taactgtaat gttttaatat
     2161 cgctggacat tactaataaa cccactataa acacatgtac atatgtatgt tttggcatac
     2221 aatgagtagt tggggaaaaa atgtgtaaaa gcaccgtgac catcacagca taaagataac
     2281 cagctgaagt atcgaatatg agtaaccccc aaattgaatc acatgccgca actgatagga
     2341 cccatggaag tacactcttc atggcgatat acaagacaca cacaagcacg aacacccagt
     2401 tgcggaggaa attctccgta aatgaaaacc caatcggcga acaattcata cccatatatg
     2461 gtaaaagttt tgaacgcgac ttgagagcgg agagcattgc ggctgataag gttttagcgc
     2521 taagcgggct ttataaaacg ggctgcggga ccagttttca tatcactacc gtttgagttc
     2581 ttgtgctgtg tggatactcc tcccgacaca aagccgctcc atcagccagc agtcgtctaa
     2641 tccagagacc ccggatccag atatcgacaa gtttgtacaa aaaagcaggc tacaaagcca
     2701 ccatggacaa gaagtactcc attgggctcg ctatcggcac aaacagcgtc ggctgggccg
     2761 tcattacgga cgagtacaag gtgccgagca aaaaattcaa agttctgggc aataccgatc
     2821 gccacagcat aaagaagaac ctcattggcg ccctcctgtt cgactccggg gagacggccg
     2881 aagccacgcg gctcaaaaga acagcacggc gcagatatac ccgcagaaag aatcggatct
     2941 gctacctgca ggagatcttt agtaatgaga tggctaaggt ggatgactct ttcttccata
     3001 ggctggagga gtcctttttg gtggaggagg ataaaaagca cgagcgccac ccaatctttg
     3061 gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc aaccatatat catctgagga
     3121 agaagcttgt agacagtact gataaggctg acttgcggtt gatctatctc gcgctggcgc
     3181 atatgatcaa atttcgggga cacttcctca tcgaggggga cctgaaccca gacaacagcg
     3241 atgtcgataa actctttatc caactggttc agacttacaa tcagcttttc gaagagaacc
     3301 cgatcaacgc atccggagtt gacgccaaag caatcctgag cgctaggctg tccaaatccc
     3361 ggcggctcga aaacctcatc gcacagctcc ctggggagaa gaagaacggc ctgtttggta
     3421 atcttatcgc cctgtcactc gggctgaccc ccaactttaa atctaacttc gacctggccg
     3481 aagatgccaa gcttcaactg agcaaagaca cctacgatga tgatctcgac aatctgctgg
     3541 cccagatcgg cgaccagtac gcagaccttt ttttggcggc aaagaacctg tcagacgcca
     3601 ttctgctgag tgatattctg cgagtgaaca cggagatcac caaagctccg ctgagcgcta
     3661 gtatgatcaa gcgctatgat gagcaccacc aagacttgac tttgctgaag gcccttgtca
     3721 gacagcaact gcctgagaag tacaaggaaa ttttcttcga tcagtctaaa aatggctacg
     3781 ccggatacat tgacggcgga gcaagccagg aggaatttta caaatttatt aagcccatct
     3841 tggaaaaaat ggacggcacc gaggagctgc tggtaaagct taacagagaa gatctgttgc
     3901 gcaaacagcg cactttcgac aatggaagca tcccccacca gattcacctg ggcgaactgc
     3961 acgctatcct caggcggcaa gaggatttct accccttttt gaaagataac agggaaaaga
     4021 ttgagaaaat cctcacattt cggataccct actatgtagg ccccctcgcc cggggaaatt
     4081 ccagattcgc gtggatgact cgcaaatcag aagagaccat cactccctgg aacttcgagg
     4141 aagtcgtgga taagggggcc tctgcccagt ccttcatcga aaggatgact aactttgata
     4201 aaaatctgcc taacgaaaag gtgcttccta aacactctct gctgtacgag tacttcacag
     4261 tttataacga gctcaccaag gtcaaatacg tcacagaagg gatgagaaag ccagcattcc
     4321 tgtctggaga gcagaagaaa gctatcgtgg acctcctctt caagacgaac cggaaagtta
     4381 ccgtgaaaca gctcaaagaa gactatttca aaaagattga atgtttcgac tctgttgaaa
     4441 tcagcggagt ggaggatcgc ttcaacgcat ccctgggaac gtatcacgat ctcctgaaaa
     4501 tcattaaaga caaggacttc ctggacaatg aggagaacga ggacattctt gaggacattg
     4561 tcctcaccct tacgttgttt gaagataggg agatgattga agaacgcttg aaaacttacg
     4621 ctcatctctt cgacgacaaa gtcatgaaac agctcaagag gcgccgatat acaggatggg
     4681 ggcggctgtc aagaaaactg atcaatggga tccgagacaa gcagagtgga aagacaatcc
     4741 tggattttct taagtccgat ggatttgcca accggaactt catgcagttg atccatgatg
     4801 actctctcac ctttaaggag gacatccaga aagcacaagt ttctggccag ggggacagtc
     4861 ttcacgagca catcgctaat cttgcaggta gcccagctat caaaaaggga atactgcaga
     4921 ccgttaaggt cgtggatgaa ctcgtcaaag taatgggaag gcataagccc gagaatatcg
     4981 ttatcgagat ggcccgagag aaccaaacta cccagaaggg acagaagaac agtagggaaa
     5041 ggatgaagag gattgaagag ggtataaaag aactggggtc ccaaatcctt aaggaacacc
     5101 cagttgaaaa cacccagctt cagaatgaga agctctacct gtactacctg cagaacggca
     5161 gggacatgta cgtggatcag gaactggaca tcaatcggct ctccgactac gacgtggctg
     5221 ctatcgtgcc ccagtctttt ctcaaagatg attctattga taataaagtg ttgacaagat
     5281 ccgataaagc tagagggaag agtgataacg tcccctcaga agaagttgtc aagaaaatga
     5341 aaaattattg gcggcagctg ctgaacgcca aactgatcac acaacggaag ttcgataatc
     5401 tgactaaggc tgaacgaggt ggcctgtctg agttggataa agccggcttc atcaaaaggc
     5461 agcttgttga gacacgccag atcaccaagc acgtggccca aattctcgat tcacgcatga
     5521 acaccaagta cgatgaaaat gacaaactga ttcgagaggt gaaagttatt actctgaagt
     5581 ctaagctggt ctcagatttc agaaaggact ttcagtttta taaggtgaga gagatcaaca
     5641 attaccacca tgcgcatgat gcctacctga atgcagtggt aggcactgca cttatcaaaa
     5701 aatatcccaa gcttgaatct gaatttgttt acggagacta taaagtgtac gatgttagga
     5761 aaatgatcgc aaagtctgag caggaaatag gcaaggccac cgctaagtac ttcttttaca
     5821 gcaatattat gaattttttc aagaccgaga ttacactggc caatggagag attcggaagc
     5881 gaccacttat cgaaacaaac ggagaaacag gagaaatcgt gtgggacaag ggtagggatt
     5941 tcgcgacagt ccggaaggtc ctgtccatgc cgcaggtgaa catcgttaaa aagaccgaag
     6001 tacagaccgg aggcttctcc aaggaaagta tcctcccgaa aaggaacagc gacaagctga
     6061 tcgcacgcaa aaaagattgg gaccccaaga aatacggcgg attcgattct cctacagtcg
     6121 cttacagtgt actggttgtg gccaaagtgg agaaagggaa gtctaaaaaa ctcaaaagcg
     6181 tcaaggaact gctgggcatc acaatcatgg agcgatcaag cttcgaaaaa aaccccatcg
     6241 actttctcga ggcgaaagga tataaagagg tcaaaaaaga cctcatcatt aagcttccca
     6301 agtactctct ctttgagctt gaaaacggcc ggaaacgaat gctcgctagt gcgggcgagc
     6361 tgcagaaagg taacgagctg gcactgccct ctaaatacgt taatttcttg tatctggcca
     6421 gccactatga aaagctcaaa gggtctcccg aagataatga gcagaagcag ctgttcgtgg
     6481 aacaacacaa acactacctt gatgagatca tcgagcaaat aagcgaattc tccaaaagag
     6541 tgatcctcgc cgacgctaac ctcgataagg tgctttctgc ttacaataag cacagggata
     6601 agcccatcag ggagcaggca gaaaacatta tccacttgtt tactctgacc aacttgggcg
     6661 cgcctgcagc cttcaagtac ttcgacacca ccatagacag aaagcggtac acctctacaa
     6721 aggaggtcct ggacgccaca ctgattcatc agtcaattac ggggctctat gaaacaagaa
     6781 tcgacctctc tcagctcggt ggagacagca gggctgaccc caagaagaag aggaaggtgg
     6841 aggccagcgg ttccggacgg gctgacgcat tggacgattt tgatctggat atgctgggaa
     6901 gtgacgccct cgatgatttt gaccttgaca tgcttggttc ggatgccctt gatgactttg
     6961 acctcgacat gctcggcagt gacgcccttg atgatttcga cctggacatg ctgattaact
     7021 ctagaagttc cggatctccg aaaaagaaac gcaaagttgg tagccagtac ctgcccgaca
     7081 ccgacgaccg gcaccggatc gaggaaaagc ggaagcggac ctacgagaca ttcaagagca
     7141 tcatgaagaa gtcccccttc agcggcccca ccgaccctag acctccacct agaagaatcg
     7201 ccgtgcccag cagatccagc gccagcgtgc caaaacctgc cccccagcct taccccttca
     7261 ccagcagcct gagcaccatc aactacgacg agttccctac catggtgttc cccagcggcc
     7321 agatctctca ggcctctgct ctggctccag cccctcctca ggtgctgcct caggctcctg
     7381 ctcctgcacc agctccagcc atggtgtctg cactggctca ggcaccagca cccgtgcctg
     7441 tgctggctcc tggacctcca caggctgtgg ctccaccagc ccctaaacct acacaggccg
     7501 gcgagggcac actgtctgaa gctctgctgc agctgcagtt cgacgacgag gatctgggag
     7561 ccctgctggg aaacagcacc gatcctgccg tgttcaccga cctggccagc gtggacaaca
     7621 gcgagttcca gcagctgctg aaccagggca tccctgtggc ccctcacacc accgagccca
     7681 tgctgatgga ataccccgag gccatcaccc ggctcgtgac aggcgctcag aggcctcctg
     7741 atccagctcc tgcccctctg ggagcaccag gcctgcctaa tggactgctg tctggcgacg
     7801 aggacttcag ctctatcgcc gatatggatt tctcagcctt gctgggctct ggcagcggca
     7861 gccgggattc cagggaaggg atgtttttgc cgaagcctga ggccggctcc gctattagtg
     7921 acgtgtttga gggccgcgag gtgtgccagc caaaacgaat ccggccattt catcctccag
     7981 gaagtccatg ggccaaccgc ccactccccg ccagcctcgc accaacacca accggtccag
     8041 tacatgagcc agtcgggtca ctgaccccgg caccagtccc tcagccactg gatccagcgc
     8101 ccgcagtgac tcccgaggcc agtcacctgt tggaggatcc cgatgaagag acgagccagg
     8161 ctgtcaaagc ccttcgggag atggccgata ctgtgattcc ccagaaggaa gaggctgcaa
     8221 tctgtggcca aatggacctt tcccatccgc ccccaagggg ccatctggat gagctgacaa
     8281 ccacacttga gtccatgacc gaggatctga acctggactc acccctgacc ccggaattga
     8341 acgagattct ggataccttc ctgaacgacg agtgcctctt gcatgccatg catatcagca
     8401 caggactgtc catcttcgac acatctctgt tttgaaaccc agctttcttg tacaaagtgg
     8461 tgagctccgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     8521 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     8581 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     8641 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
     8701 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     8761 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     8821 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     8881 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     8941 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     9001 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     9061 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     9121 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     9181 agctgtacaa gcaccggtcc acgtgacgta agctagcagg atctttgtga aggaacctta
     9241 cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct ctaaggtaaa
     9301 tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt gtgtatttta
     9361 gattccaacc tatggaactg atgaatggga gcagtggtgg aatgccttta atgaggaaaa
     9421 cctgttttgc tcagaagaaa tgccatctag tgatgatgag gctactgctg actctcaaca
     9481 ttctactcct ccaaaaaaga agagaaaggt agaagacccc aaggactttc cttcagaatt
     9541 gctaagtttt ttgagtcatg ctgtgtttag taatagaact cttgcttgct ttgctattta
     9601 caccacaaag gaaaaagctg cactgctata caagaaaatt atggaaaaat attctgtaac
     9661 ctttataagt aggcataaca gttataatca taacatactg ttttttctta ctccacacag
     9721 gcatagagtg tctgctatta ataactatgc tcaaaaattg tgtaccttta gctttttaat
     9781 ttgtaaaggg gttaataagg aatatttgat gtatagtgcc ttgactagag atcataatca
     9841 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
     9901 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
     9961 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10021 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc ccgtttaaac
    10081 tacgcgtaat tcaaacaggg ttctggcgtc gttctcgtac tgttttcccc aggccagtgc
    10141 tttagcgtta ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
    10201 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
    10261 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
    10321 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
    10381 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
    10441 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
    10501 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
    10561 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
    10621 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
    10681 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
    10741 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
    10801 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
    10861 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
    10921 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
    10981 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
    11041 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
    11101 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
    11161 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
    11221 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
    11281 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
    11341 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
    11401 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
    11461 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
    11521 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
    11581 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
    11641 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
    11701 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
    11761 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
    11821 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
    11881 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
    11941 gcgagtcagt gagcgaggaa gcggaaga
//
